TY - JOUR
T1 - The role of reactive oxygen species on Plasmodium melanotic encapsulation in Anopheles gambiae
AU - Kumar, Sanjeev
AU - Christophides, George K.
AU - Cantera, Rafael
AU - Charles, Bradley
AU - Han, Yeon Soo
AU - Meister, Stephan
AU - Dimopoulos, George
AU - Kafatos, Fotis C.
AU - Barillas-Mury, Carolina
PY - 2003/11/25
Y1 - 2003/11/25
N2 - Malaria transmission depends on the competence of some Anopheles mosquitoes to sustain Plasmodium development (susceptibility). A genetically selected refractory strain of Anopheles gambiae blocks Plasmodium development, melanizing, and encapsulating the parasite in a reaction that begins with tyrosine oxidation, and involves three quantitative trait loci. Morphological and microarray mRNA expression analysis suggest that the refractory and susceptible strains have broad physiological differences, which are related to the production and detoxification of reactive oxygen species. Physiological studies corroborate that the refractory strain is in a chronic state of oxidative stress, which is exacerbated by blood feeding, resulting in increased steady-state levels of reactive oxygen species, which favor melanization of parasites as well as Sephadex beads.
AB - Malaria transmission depends on the competence of some Anopheles mosquitoes to sustain Plasmodium development (susceptibility). A genetically selected refractory strain of Anopheles gambiae blocks Plasmodium development, melanizing, and encapsulating the parasite in a reaction that begins with tyrosine oxidation, and involves three quantitative trait loci. Morphological and microarray mRNA expression analysis suggest that the refractory and susceptible strains have broad physiological differences, which are related to the production and detoxification of reactive oxygen species. Physiological studies corroborate that the refractory strain is in a chronic state of oxidative stress, which is exacerbated by blood feeding, resulting in increased steady-state levels of reactive oxygen species, which favor melanization of parasites as well as Sephadex beads.
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U2 - 10.1073/pnas.2036262100
DO - 10.1073/pnas.2036262100
M3 - Article
C2 - 14623973
AN - SCOPUS:0344630287
SN - 0027-8424
VL - 100
SP - 14139
EP - 14144
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - SUPPL. 2
ER -