TY - JOUR
T1 - The relationship between prostaglandins and histamine in the ovulatory process as determined with the in vitro perfused rabbit ovary
AU - Kitai, H.
AU - Kobayashi, Y.
AU - Santulli, R.
AU - Wright, K. H.
AU - Wallach, E. E.
N1 - Funding Information:
Received July 2, 1984; revised and accepted November 27, 1984. *Supported by National Institutes of Health grant HD-05948, The Connelly Foundation, and The Mitchell and Lillian Duberstein Foundation. tConnelly Foundation Fellow in Reproductive Biology, Pennsylvania Hospital. :j:Department of Obstetrics and Gynecology, Pennsylvania Hospital and the University of Pennsylvania School of Medicine. §Reprint requests: Edward E. Wallach, M.D., Department of Gynecology and Obstetrics, The Johns Hopkins Medical Institutions, Baltimore, Maryland 21205.
PY - 1985
Y1 - 1985
N2 - The process of follicle rupture has been described as an inflammatory reaction in which prostaglandins (PGs) and/or histamine may be involved. With an in vitro perfused rabbit ovary preparation, experiments were carried out for determination of whether a relationship exists among PGs, histamine, and ovulation. PGF(2α) alone was capable of inducing ovulation when added to the perfusion fluid at 1, 10, and 100 ng/ml. Effectiveness in achieving ovulation varied directly with the dosage; however, the ovulatory efficiency of PGF(2α)-treated ovaries was lower than that of ovaries exposed to human chorionic gonadotropin (hCG, 100 IU). PGF(2α)-induced ovulation could not be blocked by the H2 receptor antagonist, cimetidine. The PG synthesis inhibitor, indomethacin, did not prevent histamine-induced ovulation. Ovulation induced by hCG was partially blocked by the administration of indomethacin; however, the concomitant administration of cimetidine was not associated with further reduction in ovulation. In all but one experimental group, the majority of ovulated ova did not progress beyond the intact germinal vesicle stage unless the ovaries had been exposed to hCG. On the basis of these experiments, PGs and histamine do not appear to be interdependent in their effects on the ovulatory process in vitro.
AB - The process of follicle rupture has been described as an inflammatory reaction in which prostaglandins (PGs) and/or histamine may be involved. With an in vitro perfused rabbit ovary preparation, experiments were carried out for determination of whether a relationship exists among PGs, histamine, and ovulation. PGF(2α) alone was capable of inducing ovulation when added to the perfusion fluid at 1, 10, and 100 ng/ml. Effectiveness in achieving ovulation varied directly with the dosage; however, the ovulatory efficiency of PGF(2α)-treated ovaries was lower than that of ovaries exposed to human chorionic gonadotropin (hCG, 100 IU). PGF(2α)-induced ovulation could not be blocked by the H2 receptor antagonist, cimetidine. The PG synthesis inhibitor, indomethacin, did not prevent histamine-induced ovulation. Ovulation induced by hCG was partially blocked by the administration of indomethacin; however, the concomitant administration of cimetidine was not associated with further reduction in ovulation. In all but one experimental group, the majority of ovulated ova did not progress beyond the intact germinal vesicle stage unless the ovaries had been exposed to hCG. On the basis of these experiments, PGs and histamine do not appear to be interdependent in their effects on the ovulatory process in vitro.
UR - http://www.scopus.com/inward/record.url?scp=0021883093&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021883093&partnerID=8YFLogxK
U2 - 10.1016/s0015-0282(16)48511-0
DO - 10.1016/s0015-0282(16)48511-0
M3 - Article
C2 - 3857188
AN - SCOPUS:0021883093
SN - 0015-0282
VL - 43
SP - 646
EP - 651
JO - Fertility and sterility
JF - Fertility and sterility
IS - 4
ER -