The real-time signal recording of sciatic nerve of rabbits after acute implantation of self-made intrafascicular electrodes

Objective: Multiunit recordings were made in anesthetized rabbits by microsurgery technique to discover one of the appropriate methods in the signal recording of peripheral nerve after acute implantation of intrafascicular electrodes and comparison was made with Bionic single electrode. Methods: Recording electrode fabricated from-60 μm 95% Pt-5% Ir wire (insulated but insulation removed at the end about 1.5 mm) was implanted directly by microsurgery technique into the fascicle of the sciatic nerve of rabbit, the same electrode was placed outside the fascicle and parallel to the intrafascicular electrode as reference electrode. Both distal ends were coupled to a differential amplifier and bandpass filter. Stimulating with 2 hook electrodes at the proximal points of the fascicle which the recording electrode threaded, the signal was recorded on an EMG instrument. The same method was used with Bionic single electrode as a comparison. Results: 4 of 5 legs of rabbits were successful recorded, and the signals recorded have the characters of the motor nerve. Conclusions: The real-time signal of sciatic nerve of rabbits can be recorded immediately with self-made intrafascicular electrodes implanted directly into the fascicles.