The Q system: A repressible binary system for transgene expression, lineage tracing, and mosaic analysis

Christopher J. Potter; Bosiljka Tasic; Emilie V. Russler; Liang Liang; Liqun Luo

doi:10.1016/j.cell.2010.02.025

The Q system: A repressible binary system for transgene expression, lineage tracing, and mosaic analysis

Christopher J. Potter
, Bosiljka Tasic
, Emilie V. Russler
, Liang Liang
, Liqun Luo

Research output: Contribution to journal › Article › peer-review

304 Scopus citations

Abstract

We describe a new repressible binary expression system based on the regulatory genes from the Neurospora qa gene cluster. This " Q system" offers attractive features for transgene expression in Drosophila and mammalian cells: low basal expression in the absence of the transcriptional activator QF, high QF-induced expression, and QF repression by its repressor QS. Additionally, feeding flies quinic acid can relieve QS repression. The Q system offers many applications, including (1) intersectional " logic gates" with the GAL4 system for manipulating transgene expression patterns, (2) GAL4-independent MARCM analysis, and (3) coupled MARCM analysis to independently visualize and genetically manipulate siblings from any cell division. We demonstrate the utility of the Q system in determining cell division patterns of a neuronal lineage and gene function in cell growth and proliferation, and in dissecting neurons responsible for olfactory attraction. The Q system can be expanded to other uses in Drosophila and to any organism conducive to transgenesis.

Original language	English (US)
Pages (from-to)	536-548
Number of pages	13
Journal	Cell
Volume	141
Issue number	3
DOIs	https://doi.org/10.1016/j.cell.2010.02.025
State	Published - Apr 2010
Externally published	Yes

Keywords

DNA
Molneuro

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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10.1016/j.cell.2010.02.025

Cite this

@article{be535e6ecf13408c8913bd448e8a0052,

title = "The Q system: A repressible binary system for transgene expression, lineage tracing, and mosaic analysis",

abstract = "We describe a new repressible binary expression system based on the regulatory genes from the Neurospora qa gene cluster. This {"} Q system{"} offers attractive features for transgene expression in Drosophila and mammalian cells: low basal expression in the absence of the transcriptional activator QF, high QF-induced expression, and QF repression by its repressor QS. Additionally, feeding flies quinic acid can relieve QS repression. The Q system offers many applications, including (1) intersectional {"} logic gates{"} with the GAL4 system for manipulating transgene expression patterns, (2) GAL4-independent MARCM analysis, and (3) coupled MARCM analysis to independently visualize and genetically manipulate siblings from any cell division. We demonstrate the utility of the Q system in determining cell division patterns of a neuronal lineage and gene function in cell growth and proliferation, and in dissecting neurons responsible for olfactory attraction. The Q system can be expanded to other uses in Drosophila and to any organism conducive to transgenesis.",

keywords = "DNA, Molneuro",

author = "Potter, \{Christopher J.\} and Bosiljka Tasic and Russler, \{Emilie V.\} and Liang Liang and Liqun Luo",

note = "Funding Information: We thank D. Luginbuhl, M. Tynan La Fontaine, and T. Chou for technical assistance; K. Wehner for HeLa cells and mouse anti-HA and mouse anti-fibrillarin antibodies; M. Simon for S2 cells and adult eye sectioning; T. Clandinin for 24B10 antibodies; S. Block for luminometer usage; the Fungal Genetics Stock Center for cosmids containing QF and QS; the Developmental Biology Hybridoma Bank for monoclonal antibodies; the Bloomington Stock Center for flies; and D. Berdnik, Y.-H. Chou, K. Miyamichi, M. Spletter, L. Sweeney, and W. Hong for comments on the manuscript. This research was supported by grants from the National Institutes of Health and Human Frontiers Science Program. C.J.P. and B.T. were supported by the Damon Runyon Cancer Research Foundation (C.J.P., DRG-1766-03; B.T., DRG-1819-04). L. Liang was supported by the Stanford Graduate Fellowship. L. Luo is an investigator of the Howard Hughes Medical Institute. C.J.P. performed all the Drosophila in vivo experiments, with the help of E.V.R. B.T. identified the qa system from Neurospora as a candidate binary expression system and performed the Drosophila and mammalian cultured cell experiments. L. Liang performed the quantitative analysis in Figure 5 . L. Luo supervised the project, and wrote the paper with C.J.P. and B.T. ",

year = "2010",

month = apr,

doi = "10.1016/j.cell.2010.02.025",

language = "English (US)",

volume = "141",

pages = "536--548",

journal = "Cell",

issn = "0092-8674",

publisher = "Elsevier B.V.",

number = "3",

}

TY - JOUR

T1 - The Q system

T2 - A repressible binary system for transgene expression, lineage tracing, and mosaic analysis

AU - Potter, Christopher J.

AU - Tasic, Bosiljka

AU - Russler, Emilie V.

AU - Liang, Liang

AU - Luo, Liqun

N1 - Funding Information: We thank D. Luginbuhl, M. Tynan La Fontaine, and T. Chou for technical assistance; K. Wehner for HeLa cells and mouse anti-HA and mouse anti-fibrillarin antibodies; M. Simon for S2 cells and adult eye sectioning; T. Clandinin for 24B10 antibodies; S. Block for luminometer usage; the Fungal Genetics Stock Center for cosmids containing QF and QS; the Developmental Biology Hybridoma Bank for monoclonal antibodies; the Bloomington Stock Center for flies; and D. Berdnik, Y.-H. Chou, K. Miyamichi, M. Spletter, L. Sweeney, and W. Hong for comments on the manuscript. This research was supported by grants from the National Institutes of Health and Human Frontiers Science Program. C.J.P. and B.T. were supported by the Damon Runyon Cancer Research Foundation (C.J.P., DRG-1766-03; B.T., DRG-1819-04). L. Liang was supported by the Stanford Graduate Fellowship. L. Luo is an investigator of the Howard Hughes Medical Institute. C.J.P. performed all the Drosophila in vivo experiments, with the help of E.V.R. B.T. identified the qa system from Neurospora as a candidate binary expression system and performed the Drosophila and mammalian cultured cell experiments. L. Liang performed the quantitative analysis in Figure 5 . L. Luo supervised the project, and wrote the paper with C.J.P. and B.T.

PY - 2010/4

Y1 - 2010/4

N2 - We describe a new repressible binary expression system based on the regulatory genes from the Neurospora qa gene cluster. This " Q system" offers attractive features for transgene expression in Drosophila and mammalian cells: low basal expression in the absence of the transcriptional activator QF, high QF-induced expression, and QF repression by its repressor QS. Additionally, feeding flies quinic acid can relieve QS repression. The Q system offers many applications, including (1) intersectional " logic gates" with the GAL4 system for manipulating transgene expression patterns, (2) GAL4-independent MARCM analysis, and (3) coupled MARCM analysis to independently visualize and genetically manipulate siblings from any cell division. We demonstrate the utility of the Q system in determining cell division patterns of a neuronal lineage and gene function in cell growth and proliferation, and in dissecting neurons responsible for olfactory attraction. The Q system can be expanded to other uses in Drosophila and to any organism conducive to transgenesis.

AB - We describe a new repressible binary expression system based on the regulatory genes from the Neurospora qa gene cluster. This " Q system" offers attractive features for transgene expression in Drosophila and mammalian cells: low basal expression in the absence of the transcriptional activator QF, high QF-induced expression, and QF repression by its repressor QS. Additionally, feeding flies quinic acid can relieve QS repression. The Q system offers many applications, including (1) intersectional " logic gates" with the GAL4 system for manipulating transgene expression patterns, (2) GAL4-independent MARCM analysis, and (3) coupled MARCM analysis to independently visualize and genetically manipulate siblings from any cell division. We demonstrate the utility of the Q system in determining cell division patterns of a neuronal lineage and gene function in cell growth and proliferation, and in dissecting neurons responsible for olfactory attraction. The Q system can be expanded to other uses in Drosophila and to any organism conducive to transgenesis.

KW - DNA

KW - Molneuro

UR - https://www.scopus.com/pages/publications/77951925182

UR - https://www.scopus.com/pages/publications/77951925182#tab=citedBy

U2 - 10.1016/j.cell.2010.02.025

DO - 10.1016/j.cell.2010.02.025

M3 - Article

C2 - 20434990

AN - SCOPUS:77951925182

SN - 0092-8674

VL - 141

SP - 536

EP - 548

JO - Cell

JF - Cell

IS - 3

ER -

The Q system: A repressible binary system for transgene expression, lineage tracing, and mosaic analysis

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Keywords

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