TY - JOUR
T1 - The mouse telomerase RNA 5'-end lies just upstream of the telomerase template sequence
AU - Hinkley, Craig S.
AU - Blasco, Maria A.
AU - Funk, Walter D.
AU - Feng, Junli
AU - Villeponteau, Bryant
AU - Greider, Carol W.
AU - Herr, Winship
N1 - Funding Information:
We thank W.Tansey for a critical reading of the manuscript and J.Duffy and P.Renna for artwork. This study was supported by PHS grant CA13106 from the National Cancer Institute to W.H. and C.G. and the Geron Corporation.
PY - 1998/1/15
Y1 - 1998/1/15
N2 - Telomerase is a ribonucleoprotein enzyme with an essential RNA component. Embedded within the telomerase RNA is a template sequence for telomere synthesis. We have characterized the structure of the 5' regions of the human and mouse telomerase-RNA genes, and have found a striking difference in the location of the template sequence: Whereas the 5'-end of the human telomerase RNA lies 45 nt from the telomerase-RNA template sequence, the 5'-end of the mouse telomerase RNA lies just 2 nt from the telomerase-RNA template sequence. Analysis of genomic sequences flanking the 5'-end of the human and mouse telomerase RNA-coding sequences reveals similar promoter-element arrangements typical of mRNA-type promoters: a TATA box-like element and an upstream region containing a consensus CCAAT box. This putative promoter structure contrasts with that of the ciliate telomerase-RNA genes whose structure resembles RNA polymerase III U6 small nuclear RNA (snRNA) promoters. These and other comparisons suggest that, during evolution, both the RNA-polymerase specificity of telomerase RNA-gene promoters and, more recently, the position of the template sequence in the telomerase RNA changed.
AB - Telomerase is a ribonucleoprotein enzyme with an essential RNA component. Embedded within the telomerase RNA is a template sequence for telomere synthesis. We have characterized the structure of the 5' regions of the human and mouse telomerase-RNA genes, and have found a striking difference in the location of the template sequence: Whereas the 5'-end of the human telomerase RNA lies 45 nt from the telomerase-RNA template sequence, the 5'-end of the mouse telomerase RNA lies just 2 nt from the telomerase-RNA template sequence. Analysis of genomic sequences flanking the 5'-end of the human and mouse telomerase RNA-coding sequences reveals similar promoter-element arrangements typical of mRNA-type promoters: a TATA box-like element and an upstream region containing a consensus CCAAT box. This putative promoter structure contrasts with that of the ciliate telomerase-RNA genes whose structure resembles RNA polymerase III U6 small nuclear RNA (snRNA) promoters. These and other comparisons suggest that, during evolution, both the RNA-polymerase specificity of telomerase RNA-gene promoters and, more recently, the position of the template sequence in the telomerase RNA changed.
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U2 - 10.1093/nar/26.2.532
DO - 10.1093/nar/26.2.532
M3 - Article
C2 - 9421511
AN - SCOPUS:0032518147
SN - 0305-1048
VL - 26
SP - 532
EP - 536
JO - Nucleic acids research
JF - Nucleic acids research
IS - 2
ER -