TY - JOUR
T1 - The mouse ribosomal DNA promoter has more stringent requirements in vivo than in vitro
AU - Henderson, Sheryl L.
AU - Sollner-Webb, Barbara
PY - 1990/9
Y1 - 1990/9
N2 - Using mouse ribosomal DNA templates bearing polymerase I terminators to prevent transcriptional interference (S. L. Henderson, K. Ryan, and B. Sollner-Webb, Genes Dev. 3:212-223, 1989) and facilitate promoter analysis in intact cells, we demonstrate that a -140 promoter domain (as well as the core region) is essential for appreciable levels of initiation in vivo. This in vivo polymerase I promoter can also be detected in vitro but only under very stringent conditions.
AB - Using mouse ribosomal DNA templates bearing polymerase I terminators to prevent transcriptional interference (S. L. Henderson, K. Ryan, and B. Sollner-Webb, Genes Dev. 3:212-223, 1989) and facilitate promoter analysis in intact cells, we demonstrate that a -140 promoter domain (as well as the core region) is essential for appreciable levels of initiation in vivo. This in vivo polymerase I promoter can also be detected in vitro but only under very stringent conditions.
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U2 - 10.1128/MCB.10.9.4970
DO - 10.1128/MCB.10.9.4970
M3 - Article
C2 - 2388633
AN - SCOPUS:0025161204
SN - 0270-7306
VL - 10
SP - 4970
EP - 4973
JO - Molecular and cellular biology
JF - Molecular and cellular biology
IS - 9
ER -