TY - JOUR
T1 - The membrane phospholipid binding protein annexin A2 promotes phagocytosis and nonlytic exocytosis of cryptococcus neoformans and impacts survival in fungal infection
AU - Stukes, Sabriya
AU - Coelho, Carolina
AU - Rivera, Johanna
AU - Jedlicka, Anne E.
AU - Hajjar, Katherine A.
AU - Casadevall, Arturo
N1 - Funding Information:
This work was supported by National Institutes of Health Grants 5R01A1033774, 5R37AI033142, and 5T32A107506, and Clinical and Translational Science Awards Grants 1ULITR001073-01, 1TLI1TR001072-01, and 1KL2TR001071 from the National Center for Advancing Translational Sciences.
Publisher Copyright:
Copyright © 2016 by The American Association of Immunologists, Inc.
PY - 2016/8/15
Y1 - 2016/8/15
N2 - Cryptococcus neoformans is a fungal pathogen with a unique intracellular pathogenic strategy that includes nonlytic exocytosis, a phenomenon whereby fungal cells are expunged from macrophages without lysing the host cell. The exact mechanism and specific proteins involved in this process have yet to be completely defined. Using murine macrophages deficient in the membrane phospholipid binding protein, annexin A2 (ANXA2), we observed a significant decrease in both phagocytosis of yeast cells and the frequency of nonlytic exocytosis. Cryptococcal cells isolated from Anxa2-deficient (Anxa2-/-) bone marrow-derived macrophages and lung parenchyma displayed significantly larger capsules than those isolated from wild-type macrophages and tissues. Concomitantly, we observed significant differences in the amount of reactive oxygen species produced between Anxa2-/- and Anxa2+/+ macrophages. Despite comparable fungal burden, Anxa2-/- mice died more rapidly than wild-type mice when infected with C. neoformans, and Anxa2-/- mice exhibited enhanced inflammatory responses, suggesting that the reduced survival reflected greater immune-mediated damage. Together, these findings suggest a role for ANXA2 in the control of cryptococcal infection, macrophage function, and fungal morphology.
AB - Cryptococcus neoformans is a fungal pathogen with a unique intracellular pathogenic strategy that includes nonlytic exocytosis, a phenomenon whereby fungal cells are expunged from macrophages without lysing the host cell. The exact mechanism and specific proteins involved in this process have yet to be completely defined. Using murine macrophages deficient in the membrane phospholipid binding protein, annexin A2 (ANXA2), we observed a significant decrease in both phagocytosis of yeast cells and the frequency of nonlytic exocytosis. Cryptococcal cells isolated from Anxa2-deficient (Anxa2-/-) bone marrow-derived macrophages and lung parenchyma displayed significantly larger capsules than those isolated from wild-type macrophages and tissues. Concomitantly, we observed significant differences in the amount of reactive oxygen species produced between Anxa2-/- and Anxa2+/+ macrophages. Despite comparable fungal burden, Anxa2-/- mice died more rapidly than wild-type mice when infected with C. neoformans, and Anxa2-/- mice exhibited enhanced inflammatory responses, suggesting that the reduced survival reflected greater immune-mediated damage. Together, these findings suggest a role for ANXA2 in the control of cryptococcal infection, macrophage function, and fungal morphology.
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U2 - 10.4049/jimmunol.1501855
DO - 10.4049/jimmunol.1501855
M3 - Article
C2 - 27371724
AN - SCOPUS:84983751652
SN - 0022-1767
VL - 197
SP - 1252
EP - 1261
JO - Journal of Immunology
JF - Journal of Immunology
IS - 4
ER -