The kinesin-like protein KLP61F is essential for mitosis in Drosophila

Margarete M S Heck, Andrea Pereira, Patty Pesavento, Yvonne Yannoni, Allan C. Spradling, Lawrence S B Goldstein

Research output: Contribution to journalArticlepeer-review

240 Scopus citations


We report here that disruption of a recently discovered kinesin-like protein in Drosophila melanogaster, KLP61F, results in a mitotic mutation lethal to the organism. We show that in the absence of KLP61F function, spindle poles fail to separate, resulting in the formation of monopolar mitotic spindles. The resulting phenotype of metaphase arrest with polyploid cells is reminiscent of that seen in the fungal bimC and cut7 mutations, where it has also been shown that spindle pole bodies are not segregated. KLP61F is specifically expressed in proliferating tissues during embryonic and larval development, consistent with a primary role in cell division. The structural and functional homology of the KLP61F, bimC, cut7, and Eg5 kinesin-like proteins demonstrates the existence of a conserved family of kinesin-like molecules important for spindle pole separation and mitotic spindle dynamics.

Original languageEnglish (US)
Pages (from-to)665-679
Number of pages15
JournalJournal of Cell Biology
Issue number3
StatePublished - Nov 1993

ASJC Scopus subject areas

  • Cell Biology


Dive into the research topics of 'The kinesin-like protein KLP61F is essential for mitosis in Drosophila'. Together they form a unique fingerprint.

Cite this