The Jak-STAT target chinmo prevents sex transformation of adult stem cells in the drosophila testis niche

Qing Ma; Matthew Wawersik; Erika L. Matunis

doi:10.1016/j.devcel.2014.10.004

The Jak-STAT target chinmo prevents sex transformation of adult stem cells in the drosophila testis niche

Qing Ma, Matthew Wawersik, Erika L. Matunis

School of Medicine

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

Local signals maintain adult stem cells in many tissues. Whether the sexual identity of adult stem cells must also be maintained was not known. In the adult Drosophila testis niche, local Jak-STAT signaling promotes somatic cyst stem cell (CySC) renewal through several effectors, including the putative transcription factor Chronologically inappropriate morphogenesis (Chinmo). Here, we find that Chinmo also prevents feminization of CySCs. Chinmo promotes expression of the canonical male sex determination factor DoublesexM (Dsx^M) within CySCs and their progeny, and ectopic expression of DsxM in the CySC lineage partially rescues the chinmo sex transformation phenotype, placing Chinmo upstream of Dsx^M. The Dsx homolog DMRT1 prevents the male-to-female conversion of differentiated somatic cells in the adult mammalian testis, but its regulation is not well understood. Our work indicates that sex maintenance occurs in adult somatic stem cells and that this highly conserved process is governed by effectors of niche signals.

Original language	English (US)
Pages (from-to)	474-486
Number of pages	13
Journal	Developmental Cell
Volume	31
Issue number	4
DOIs	https://doi.org/10.1016/j.devcel.2014.10.004
State	Published - Nov 24 2014

ASJC Scopus subject areas

Molecular Biology
Biochemistry, Genetics and Molecular Biology(all)
Developmental Biology
Cell Biology

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10.1016/j.devcel.2014.10.004

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title = "The Jak-STAT target chinmo prevents sex transformation of adult stem cells in the drosophila testis niche",

abstract = "Local signals maintain adult stem cells in many tissues. Whether the sexual identity of adult stem cells must also be maintained was not known. In the adult Drosophila testis niche, local Jak-STAT signaling promotes somatic cyst stem cell (CySC) renewal through several effectors, including the putative transcription factor Chronologically inappropriate morphogenesis (Chinmo). Here, we find that Chinmo also prevents feminization of CySCs. Chinmo promotes expression of the canonical male sex determination factor DoublesexM (DsxM) within CySCs and their progeny, and ectopic expression of DsxM in the CySC lineage partially rescues the chinmo sex transformation phenotype, placing Chinmo upstream of DsxM. The Dsx homolog DMRT1 prevents the male-to-female conversion of differentiated somatic cells in the adult mammalian testis, but its regulation is not well understood. Our work indicates that sex maintenance occurs in adult somatic stem cells and that this highly conserved process is governed by effectors of niche signals.",

author = "Qing Ma and Matthew Wawersik and Matunis, {Erika L.}",

note = "Funding Information: We thank Helen Salz and Amir Oryan for helpful discussions, Margaret de Cuevas, Leah Greenspan, David Zarkower, and Geraldine Seydoux for comments on the manuscript, Yijie Li for help with dissections, and Nick Sokol, Brian Oliver, Gyunghee Lee, Denise Montell, and Xiaobo Wang for flies or antisera. This work was funded by the NIH (HD040307 and HD052937) (to E.L.M.). Publisher Copyright: {\textcopyright} 2014 Elsevier Inc.",

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N2 - Local signals maintain adult stem cells in many tissues. Whether the sexual identity of adult stem cells must also be maintained was not known. In the adult Drosophila testis niche, local Jak-STAT signaling promotes somatic cyst stem cell (CySC) renewal through several effectors, including the putative transcription factor Chronologically inappropriate morphogenesis (Chinmo). Here, we find that Chinmo also prevents feminization of CySCs. Chinmo promotes expression of the canonical male sex determination factor DoublesexM (DsxM) within CySCs and their progeny, and ectopic expression of DsxM in the CySC lineage partially rescues the chinmo sex transformation phenotype, placing Chinmo upstream of DsxM. The Dsx homolog DMRT1 prevents the male-to-female conversion of differentiated somatic cells in the adult mammalian testis, but its regulation is not well understood. Our work indicates that sex maintenance occurs in adult somatic stem cells and that this highly conserved process is governed by effectors of niche signals.

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The Jak-STAT target chinmo prevents sex transformation of adult stem cells in the drosophila testis niche

Abstract

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