The introduction of radioisotopes into RNA by methylation in vitro

Kirby D. Smith, John L. Armstrong, Brian J. McCarthy

Research output: Contribution to journalArticlepeer-review

47 Scopus citations


Either 3H or 14C may be introduced at high specific radioactivity into RNA in vitro by treatment with dimethyl sulfate. The procedure is rapid and simple and no degradation of the RNA occurs. The hybridization properties of the methylated RNA compare with those of molecules randomly labeled in vivo. The method is of potential value in cases where low metabolic activity or other biological parameters preclude labeling of RNA in vivo during synthesis.

Original languageEnglish (US)
Pages (from-to)323-330
Number of pages8
JournalBBA Section Nucleic Acids And Protein Synthesis
Issue number2
StatePublished - Jul 18 1967
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)


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