The in vitro effect of trimethoprim on blastogenic transformation of human peripheral blood lymphocytes

Howard J. Rogers, Paul S. Lietman

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Trimethoprim (TP) at concentrations of 1 × 10-6 to 1 × 10-3 M inhibits the incorporation of thymidine, uridine, formate, and leucine by human lymphocytes undergoing blastogenic transformation induced by conconavalin A. TP does not alter cell viability, as measured by trypan blue exclusion, or cell numbers. An effect of TP on dihydrofolate reductase, such as is seen with methotrexate, is insufficient to explain the inhibitory effects of TP since an in vivo estimation of dihydrofolate reductase activity, utilizing deoxyuridine incorporation into deoxyribonucleic acid, is not inhibited. Also the observed inhibition of precursor incorporation is not fully reversed by either folate or folinate. TP did not alter the percentage of cells transforming but reduced the number of autoradiographically-labelled lymphoblasts. Immunosuppression by TP in vivo may be associated with these findings.

Original languageEnglish (US)
Pages (from-to)1592-1599
Number of pages8
JournalBiochemical Pharmacology
Volume26
Issue number17
DOIs
StatePublished - Sep 1 1977
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Pharmacology

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