@article{b1f20f28c7f242d091f1b13791365c1a,
title = "The hypoxic regulator of sterol synthesis Nro1 is a nuclear import adaptor",
abstract = "Fission yeast protein Sre1, the homolog of the mammalian sterol regulatory element-binding protein (SREBP), is a hypoxic transcription factor required for sterol homeostasis and low-oxygen growth. Nro1 regulates the stability of the N-terminal transcription factor domain of Sre1 (Sre1N) by inhibiting the action of the prolyl 4-hydroxylase-like Ofd1 in an oxygen-dependent manner. The crystal structure of Nro1 determined at 2.2 {\AA} resolution shows an all-α-helical fold that can be divided into two domains: a small N-terminal domain, and a larger C-terminal HEAT-repeat domain. Follow-up studies showed that Nro1 defines a new class of nuclear import adaptor that functions both in Ofd1 nuclear localization and in the oxygen-dependent inhibition of Ofd1 to control the hypoxic response.",
author = "Yeh, {Tzu Lan} and Lee, {Chih Yung S.} and Amzel, {L. Mario} and Espenshade, {Peter J.} and Bianchet, {Mario A.}",
note = "Funding Information: We acknowledge the use of beamlines X4A, X4C, and X6A at the Brookhaven National Laboratory, and the use of the SGX Collaborative Access Team (SGX-CAT) beamline facilities at Sector 31 of the Advanced Photon Source at Argonne National Laboratory that was provided by Eli Lilly & Company that operates the facility, supported by the U. S. Department of Energy, Office of Science, Office of Basic Energy Sciences, under Contract No. DE-AC02-06CH11357. Thanks to the Taplin Biological Mass Spectrometry Facility (Harvard Medical School) for mass spectrometry data and to Tony Hunter (Salk Institute) for strains and plasmids. This work was supported by NIH grants HL-077588 (P.J.E.) and ARRA-1RO1NS061827 (L.M.A.), in addition to an Established Investigator Award from the American Heart Association to P.J.E. and American Heart Association Predoctoral Fellowship to C.-Y.S.L. ",
year = "2011",
month = apr,
day = "13",
doi = "10.1016/j.str.2011.01.017",
language = "English (US)",
volume = "19",
pages = "503--514",
journal = "Structure",
issn = "0969-2126",
publisher = "Cell Press",
number = "4",
}