TY - JOUR
T1 - The homology of cortical microtubules in platyhelminth spermatozoa
T2 - A comparative immunocytochemical study of acetylated tubulin
AU - Justine, Jean Lou
AU - Iomini, Carlo
AU - Raikova, Olga I.
AU - Mollaret, Isabelle
N1 - Funding Information:
Eels were provided by Dr C. Salmon, Laboratory of Comparative Physiology, MNHN. Cavernicolous shrimps were provided by Fulvio Gasparo. Dr A. Fournier and S. Trouvé (University of Perpignan) provided cercariae of Echinostoma caproni. The C105 antibody was provided by Prof. J. M. Andreu, Centro de Investigaciones Biologicas, CSIC, Madrid. Prof. M. Ferraguti helped for the study of Troglocaridicola sp. Prof. B. G. M. Jamieson kindly edited the English. This work was partly supported by a BQR “Immunocytochimie des spermatozoı̈des de Plathelminthes et Nématodes” from the Muséum, the INTAS grant no. 93-2176, “Ultrastructure and immunocytochemistry of the cytoskeleton of spermatozoa, eggs and fertilization in selected invertebrates species, for the understanding of Phylogeny”, and an international grant from the French Embassy in Australia.
PY - 1998/7
Y1 - 1998/7
N2 - Spermatozoa of certain acoels, a group of primitive Platyhelminthes, and spermatozoa of the most derived Platyhelminthes, the Cercomeridea (parasitic Platyhelminthes), show a general morphological resemblance in that they are long filiform cells with two incorporated axonemes and longitudinal cortical microtubules. A possible way to test the homology of these cortical microtubules in the different groups is to analyze the presence/absence of post-translational modifications of tubulin. An indirect immunofluorescence study showed that the doublet microtubules of the sperm axonemes are labelled by an anti acetylated-alpha-tubulin antibody in all groups, irrespective of the axoneme pattern (9 + 0, 9 + 2 and non-trepaxonematan 9 + '1' in various acoels, and trepaxonematan 9 + '1' of the temnocephalid Troglocaridicola sp., the digenean Echinostoma caproni and the monopisthocotylean monogenean Pseudodactylogyrus sp.). Significant differences are found in the sperm cortical microtubules: they are acetylated in the acoel Actinoposthia beklemischevi, but not in the digenean E. caproni and the temnocephalid Troglocaridicola sp. These results suggest that the sperm cortical microtubules of the acoels are not homologous with the morphologically similar elements found in the higher Platyhelminthes.
AB - Spermatozoa of certain acoels, a group of primitive Platyhelminthes, and spermatozoa of the most derived Platyhelminthes, the Cercomeridea (parasitic Platyhelminthes), show a general morphological resemblance in that they are long filiform cells with two incorporated axonemes and longitudinal cortical microtubules. A possible way to test the homology of these cortical microtubules in the different groups is to analyze the presence/absence of post-translational modifications of tubulin. An indirect immunofluorescence study showed that the doublet microtubules of the sperm axonemes are labelled by an anti acetylated-alpha-tubulin antibody in all groups, irrespective of the axoneme pattern (9 + 0, 9 + 2 and non-trepaxonematan 9 + '1' in various acoels, and trepaxonematan 9 + '1' of the temnocephalid Troglocaridicola sp., the digenean Echinostoma caproni and the monopisthocotylean monogenean Pseudodactylogyrus sp.). Significant differences are found in the sperm cortical microtubules: they are acetylated in the acoel Actinoposthia beklemischevi, but not in the digenean E. caproni and the temnocephalid Troglocaridicola sp. These results suggest that the sperm cortical microtubules of the acoels are not homologous with the morphologically similar elements found in the higher Platyhelminthes.
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U2 - 10.1111/j.1463-6395.1998.tb01161.x
DO - 10.1111/j.1463-6395.1998.tb01161.x
M3 - Article
AN - SCOPUS:0031815882
SN - 0001-7272
VL - 79
SP - 235
EP - 241
JO - Acta Zoologica
JF - Acta Zoologica
IS - 3
ER -