The hermes transposon of Musca domestica is an efficient tool for the mutagenesis of Schizosaccharomyces pombe

Adam G. Evertts; Christopher Plymire; Nancy L. Craig; Henry L. Levin

doi:10.1534/genetics.107.081075

The hermes transposon of Musca domestica is an efficient tool for the mutagenesis of Schizosaccharomyces pombe

Adam G. Evertts, Christopher Plymire, Nancy L. Craig, Henry L. Levin

School of Medicine

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

Currently, no transposon-based method for the mutagenesis of Schizosaccharomyces pombe exists. We have developed such a system based on the introduction of the hermes transposon from the housefly into S. pombe. This system efficiently disrupts open reading frames and allows the insertion sites to be readily identified.

Original language	English (US)
Pages (from-to)	2519-2523
Number of pages	5
Journal	Genetics
Volume	177
Issue number	4
DOIs	https://doi.org/10.1534/genetics.107.081075
State	Published - Dec 2007

ASJC Scopus subject areas

Genetics

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10.1534/genetics.107.081075

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title = "The hermes transposon of Musca domestica is an efficient tool for the mutagenesis of Schizosaccharomyces pombe",

abstract = "Currently, no transposon-based method for the mutagenesis of Schizosaccharomyces pombe exists. We have developed such a system based on the introduction of the hermes transposon from the housefly into S. pombe. This system efficiently disrupts open reading frames and allows the insertion sites to be readily identified.",

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AU - Plymire, Christopher

AU - Craig, Nancy L.

AU - Levin, Henry L.

PY - 2007/12

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AB - Currently, no transposon-based method for the mutagenesis of Schizosaccharomyces pombe exists. We have developed such a system based on the introduction of the hermes transposon from the housefly into S. pombe. This system efficiently disrupts open reading frames and allows the insertion sites to be readily identified.

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The hermes transposon of Musca domestica is an efficient tool for the mutagenesis of Schizosaccharomyces pombe

Abstract

ASJC Scopus subject areas

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