The genome sequence of the plant pathogen Xylella fastidiosa: The Xylella fastidiosa consortium of the organization for nucleotide sequencing and analysis, Sao Paulo, Brazil

A. J.G. Simpson; F. C. Reinach; P. Arruda; F. A. Abreu; M. Acencio; R. Alvarenga; L. M.C. Alves; J. E. Araya; G. S. Baia; C. S. Baptista; M. H. Barros; E. D. Bonaccorsi; S. Bordin; J. M. Bové; M. R.S. Briones; M. R.P. Bueno; A. A. Camargo; L. E.A. Camargo; D. M. Carraro; H. Carrer; N. B. Colauto; C. Colombo; F. F. Costa; M. C.R. Costa; C. M. Costa-Neto; L. L. Coutinho; M. Cristofani; E. Dias-Neto; C. Docena; H. El-Dorry; A. P. Facincani; A. J.S. Ferreira; V. C.A. Ferreira; J. A. Ferro; J. S. Fraga; S. C. França; M. C. Franco; M. Frohme; L. R. Furlan; M. Garnier; G. H. Goldman; M. H.S. Goldman; S. L. Gomes; A. Gruber; P. L. Ho; J. D. Hoheisel; M. L. Junqueira; E. L. Kemper; J. P. Kitajima; J. E. Krieger; E. E. Kuramae; F. Laigret; M. R. Lambais; L. C.C. Leite; E. G.M. Lemos; M. V.F. Lemos; S. A. Lopes; C. R. Lopes; J. A. Machado; M. A. Machado; A. M.B.N. Madeira; H. M.F. Madeira; C. L. Marino; M. V. Marques; E. A.L. Martins; E. M.F. Martins; A. Y. Matsukuma; C. F.M. Menck; E. C. Miracca; C. Y. Miyaki; C. B. Monteiro-Vitorello; D. H. Moon; M. A. Nagai; A. L.T.O. Nascimento; L. E.S. Netto; A. Nhani; F. G. Nobrega; L. R. Nunes; M. A. Oliveira; M. C. de Oliveira; R. C. de Oliveira; D. A. Palmieri; A. Paris; B. R. Peixoto; G. A.G. Pereira; H. A. Pereira; J. B. Pesquero; R. B. Quaggio; P. G. Roberto; V. Rodrigues; A. J.M. Rosa; V. E. de Rosa; R. G. de Sá; R. V. Santelli; H. E. Sawasaki; A. C.R. da Silva; A. M. da Silva; F. R. da Silva; W. A. Silva; J. F. da Silveira; M. L.Z. Silvestri; W. J. Siqueira; A. A. de Souza; A. P. de Souza; M. F. Terenzi; D. Truffi; S. M. Tsai; M. H. Tsuhako; H. Vallada; M. A. Van Sluys; S. Verjovski-Almeida; A. L. Vettore; M. A. Zago; M. Zatz; J. Meidanis; J. C. Setubal

doi:10.1038/35018003

The genome sequence of the plant pathogen Xylella fastidiosa: The Xylella fastidiosa consortium of the organization for nucleotide sequencing and analysis, Sao Paulo, Brazil

A. J.G. Simpson, F. C. Reinach, P. Arruda, F. A. Abreu, M. Acencio, R. Alvarenga, L. M.C. Alves, J. E. Araya, G. S. Baia, C. S. Baptista, M. H. Barros, E. D. Bonaccorsi, S. Bordin, J. M. Bové, M. R.S. Briones, M. R.P. Bueno, A. A. Camargo, L. E.A. Camargo, D. M. Carraro, H. CarrerN. B. Colauto, C. Colombo, F. F. Costa, M. C.R. Costa, C. M. Costa-Neto, L. L. Coutinho, M. Cristofani, E. Dias-Neto, C. Docena, H. El-Dorry, A. P. Facincani, A. J.S. Ferreira, V. C.A. Ferreira, J. A. Ferro, J. S. Fraga, S. C. França, M. C. Franco, M. Frohme, L. R. Furlan, M. Garnier, G. H. Goldman, M. H.S. Goldman, S. L. Gomes, A. Gruber, P. L. Ho, J. D. Hoheisel, M. L. Junqueira, E. L. Kemper, J. P. Kitajima, J. E. Krieger, E. E. Kuramae, F. Laigret, M. R. Lambais, L. C.C. Leite, E. G.M. Lemos, M. V.F. Lemos, S. A. Lopes, C. R. Lopes, J. A. Machado, M. A. Machado, A. M.B.N. Madeira, H. M.F. Madeira, C. L. Marino, M. V. Marques, E. A.L. Martins, E. M.F. Martins, A. Y. Matsukuma, C. F.M. Menck, E. C. Miracca, C. Y. Miyaki, C. B. Monteiro-Vitorello, D. H. Moon, M. A. Nagai, A. L.T.O. Nascimento, L. E.S. Netto, A. Nhani, F. G. Nobrega, L. R. Nunes, M. A. Oliveira, M. C. de Oliveira, R. C. de Oliveira, D. A. Palmieri, A. Paris, B. R. Peixoto, G. A.G. Pereira, H. A. Pereira, J. B. Pesquero, R. B. Quaggio, P. G. Roberto, V. Rodrigues, A. J.M. Rosa, V. E. de Rosa, R. G. de Sá, R. V. Santelli, H. E. Sawasaki, A. C.R. da Silva, A. M. da Silva, F. R. da Silva, W. A. Silva, J. F. da Silveira, M. L.Z. Silvestri, W. J. Siqueira, A. A. de Souza, A. P. de Souza, M. F. Terenzi, D. Truffi, S. M. Tsai, M. H. Tsuhako, H. Vallada, M. A. Van Sluys, S. Verjovski-Almeida, A. L. Vettore, M. A. Zago, M. Zatz, J. Meidanis, J. C. Setubal

Research output: Contribution to journal › Review article › peer-review

677 Scopus citations

Abstract

Xylella fastidiosa is a fastidious, xylem-limited bacterium that causes a range of economically important plant diseases. Here we report the complete genome sequence of X. fastidiosa clone 9a5c, which causes citrus variegated chlorosis - a serious disease of orange trees. The genome comprises a 52.7% GC-rich 2,679,305-base-pair (bp) circular chromosome and 'two plasmids of 51,158 bp and 1,285 bp. We can assign putative functions to47% of the 2,904 predicted coding regions. Efficient metabolic functions are predicted, with sugars as the principal energy and carbon source, supporting existence in the nutrient-poor xylem sap. The mechanisms associated with pathogenicity and virulence involve toxins, antibiotics and ion sequestration systems, as well as bacterium-bacterium and bacterium-host interactions mediated by a range of proteins. Orthologues of some of these proteins have only been identified in animal and human pathogens; their presence in X. fastidiosa indicates that the molecular basis for bacterial pathogenicity is both conserved and independent of host. At least 83 genes are bacteriophage-derived and include virulence-associated genes from other bacteria, providing direct evidence of phage-mediated horizontal gene transfer.

Original language	English (US)
Pages (from-to)	151-157
Number of pages	7
Journal	Nature
Volume	406
Issue number	6792
DOIs	https://doi.org/10.1038/35018003
State	Published - Jul 13 2000
Externally published	Yes

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Simpson, A. J. G., Reinach, F. C., Arruda, P., Abreu, F. A., Acencio, M., Alvarenga, R., Alves, L. M. C., Araya, J. E., Baia, G. S., Baptista, C. S., Barros, M. H., Bonaccorsi, E. D., Bordin, S., Bové, J. M., Briones, M. R. S., Bueno, M. R. P., Camargo, A. A., Camargo, L. E. A., Carraro, D. M., ... Setubal, J. C. (2000). The genome sequence of the plant pathogen Xylella fastidiosa: The Xylella fastidiosa consortium of the organization for nucleotide sequencing and analysis, Sao Paulo, Brazil. Nature, 406(6792), 151-157. https://doi.org/10.1038/35018003

Simpson, AJG, Reinach, FC, Arruda, P, Abreu, FA, Acencio, M, Alvarenga, R, Alves, LMC, Araya, JE, Baia, GS, Baptista, CS, Barros, MH, Bonaccorsi, ED, Bordin, S, Bové, JM, Briones, MRS, Bueno, MRP, Camargo, AA, Camargo, LEA, Carraro, DM, Carrer, H, Colauto, NB, Colombo, C, Costa, FF, Costa, MCR, Costa-Neto, CM, Coutinho, LL, Cristofani, M, Dias-Neto, E, Docena, C, El-Dorry, H, Facincani, AP, Ferreira, AJS, Ferreira, VCA, Ferro, JA, Fraga, JS, França, SC, Franco, MC, Frohme, M, Furlan, LR, Garnier, M, Goldman, GH, Goldman, MHS, Gomes, SL, Gruber, A, Ho, PL, Hoheisel, JD, Junqueira, ML, Kemper, EL, Kitajima, JP, Krieger, JE, Kuramae, EE, Laigret, F, Lambais, MR, Leite, LCC, Lemos, EGM, Lemos, MVF, Lopes, SA, Lopes, CR, Machado, JA, Machado, MA, Madeira, AMBN, Madeira, HMF, Marino, CL, Marques, MV, Martins, EAL, Martins, EMF, Matsukuma, AY, Menck, CFM, Miracca, EC, Miyaki, CY, Monteiro-Vitorello, CB, Moon, DH, Nagai, MA, Nascimento, ALTO, Netto, LES, Nhani, A, Nobrega, FG, Nunes, LR, Oliveira, MA, de Oliveira, MC, de Oliveira, RC, Palmieri, DA, Paris, A, Peixoto, BR, Pereira, GAG, Pereira, HA, Pesquero, JB, Quaggio, RB, Roberto, PG, Rodrigues, V, Rosa, AJM, de Rosa, VE, de Sá, RG, Santelli, RV, Sawasaki, HE, da Silva, ACR, da Silva, AM, da Silva, FR, Silva, WA, da Silveira, JF, Silvestri, MLZ, Siqueira, WJ, de Souza, AA, de Souza, AP, Terenzi, MF, Truffi, D, Tsai, SM, Tsuhako, MH, Vallada, H, Van Sluys, MA, Verjovski-Almeida, S, Vettore, AL, Zago, MA, Zatz, M, Meidanis, J & Setubal, JC 2000, 'The genome sequence of the plant pathogen Xylella fastidiosa: The Xylella fastidiosa consortium of the organization for nucleotide sequencing and analysis, Sao Paulo, Brazil', Nature, vol. 406, no. 6792, pp. 151-157. https://doi.org/10.1038/35018003

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title = "The genome sequence of the plant pathogen Xylella fastidiosa: The Xylella fastidiosa consortium of the organization for nucleotide sequencing and analysis, Sao Paulo, Brazil",

abstract = "Xylella fastidiosa is a fastidious, xylem-limited bacterium that causes a range of economically important plant diseases. Here we report the complete genome sequence of X. fastidiosa clone 9a5c, which causes citrus variegated chlorosis - a serious disease of orange trees. The genome comprises a 52.7% GC-rich 2,679,305-base-pair (bp) circular chromosome and 'two plasmids of 51,158 bp and 1,285 bp. We can assign putative functions to47% of the 2,904 predicted coding regions. Efficient metabolic functions are predicted, with sugars as the principal energy and carbon source, supporting existence in the nutrient-poor xylem sap. The mechanisms associated with pathogenicity and virulence involve toxins, antibiotics and ion sequestration systems, as well as bacterium-bacterium and bacterium-host interactions mediated by a range of proteins. Orthologues of some of these proteins have only been identified in animal and human pathogens; their presence in X. fastidiosa indicates that the molecular basis for bacterial pathogenicity is both conserved and independent of host. At least 83 genes are bacteriophage-derived and include virulence-associated genes from other bacteria, providing direct evidence of phage-mediated horizontal gene transfer.",

author = "Simpson, {A. J.G.} and Reinach, {F. C.} and P. Arruda and Abreu, {F. A.} and M. Acencio and R. Alvarenga and Alves, {L. M.C.} and Araya, {J. E.} and Baia, {G. S.} and Baptista, {C. S.} and Barros, {M. H.} and Bonaccorsi, {E. D.} and S. Bordin and Bov{\'e}, {J. M.} and Briones, {M. R.S.} and Bueno, {M. R.P.} and Camargo, {A. A.} and Camargo, {L. E.A.} and Carraro, {D. M.} and H. Carrer and Colauto, {N. B.} and C. Colombo and Costa, {F. F.} and Costa, {M. C.R.} and Costa-Neto, {C. M.} and Coutinho, {L. L.} and M. Cristofani and E. Dias-Neto and C. Docena and H. El-Dorry and Facincani, {A. P.} and Ferreira, {A. J.S.} and Ferreira, {V. C.A.} and Ferro, {J. A.} and Fraga, {J. S.} and Fran{\c c}a, {S. C.} and Franco, {M. C.} and M. Frohme and Furlan, {L. R.} and M. Garnier and Goldman, {G. H.} and Goldman, {M. H.S.} and Gomes, {S. L.} and A. Gruber and Ho, {P. L.} and Hoheisel, {J. D.} and Junqueira, {M. L.} and Kemper, {E. L.} and Kitajima, {J. P.} and Krieger, {J. E.} and Kuramae, {E. E.} and F. Laigret and Lambais, {M. R.} and Leite, {L. C.C.} and Lemos, {E. G.M.} and Lemos, {M. V.F.} and Lopes, {S. A.} and Lopes, {C. R.} and Machado, {J. A.} and Machado, {M. A.} and Madeira, {A. M.B.N.} and Madeira, {H. M.F.} and Marino, {C. L.} and Marques, {M. V.} and Martins, {E. A.L.} and Martins, {E. M.F.} and Matsukuma, {A. Y.} and Menck, {C. F.M.} and Miracca, {E. C.} and Miyaki, {C. Y.} and Monteiro-Vitorello, {C. B.} and Moon, {D. H.} and Nagai, {M. A.} and Nascimento, {A. L.T.O.} and Netto, {L. E.S.} and A. Nhani and Nobrega, {F. G.} and Nunes, {L. R.} and Oliveira, {M. A.} and {de Oliveira}, {M. C.} and {de Oliveira}, {R. C.} and Palmieri, {D. A.} and A. Paris and Peixoto, {B. R.} and Pereira, {G. A.G.} and Pereira, {H. A.} and Pesquero, {J. B.} and Quaggio, {R. B.} and Roberto, {P. G.} and V. Rodrigues and Rosa, {A. J.M.} and {de Rosa}, {V. E.} and {de S{\'a}}, {R. G.} and Santelli, {R. V.} and Sawasaki, {H. E.} and {da Silva}, {A. C.R.} and {da Silva}, {A. M.} and {da Silva}, {F. R.} and Silva, {W. A.} and {da Silveira}, {J. F.} and Silvestri, {M. L.Z.} and Siqueira, {W. J.} and {de Souza}, {A. A.} and {de Souza}, {A. P.} and Terenzi, {M. F.} and D. Truffi and Tsai, {S. M.} and Tsuhako, {M. H.} and H. Vallada and {Van Sluys}, {M. A.} and S. Verjovski-Almeida and Vettore, {A. L.} and Zago, {M. A.} and M. Zatz and J. Meidanis and Setubal, {J. C.}",

year = "2000",

month = jul,

day = "13",

doi = "10.1038/35018003",

language = "English (US)",

volume = "406",

pages = "151--157",

journal = "Nature",

issn = "0028-0836",

publisher = "Nature Publishing Group",

number = "6792",

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TY - JOUR

T1 - The genome sequence of the plant pathogen Xylella fastidiosa

T2 - The Xylella fastidiosa consortium of the organization for nucleotide sequencing and analysis, Sao Paulo, Brazil

AU - Simpson, A. J.G.

AU - Reinach, F. C.

AU - Arruda, P.

AU - Abreu, F. A.

AU - Acencio, M.

AU - Alvarenga, R.

AU - Alves, L. M.C.

AU - Araya, J. E.

AU - Baia, G. S.

AU - Baptista, C. S.

AU - Barros, M. H.

AU - Bonaccorsi, E. D.

AU - Bordin, S.

AU - Bové, J. M.

AU - Briones, M. R.S.

AU - Bueno, M. R.P.

AU - Camargo, A. A.

AU - Camargo, L. E.A.

AU - Carraro, D. M.

AU - Carrer, H.

AU - Colauto, N. B.

AU - Colombo, C.

AU - Costa, F. F.

AU - Costa, M. C.R.

AU - Costa-Neto, C. M.

AU - Coutinho, L. L.

AU - Cristofani, M.

AU - Dias-Neto, E.

AU - Docena, C.

AU - El-Dorry, H.

AU - Facincani, A. P.

AU - Ferreira, A. J.S.

AU - Ferreira, V. C.A.

AU - Ferro, J. A.

AU - Fraga, J. S.

AU - França, S. C.

AU - Franco, M. C.

AU - Frohme, M.

AU - Furlan, L. R.

AU - Garnier, M.

AU - Goldman, G. H.

AU - Goldman, M. H.S.

AU - Gomes, S. L.

AU - Gruber, A.

AU - Ho, P. L.

AU - Hoheisel, J. D.

AU - Junqueira, M. L.

AU - Kemper, E. L.

AU - Kitajima, J. P.

AU - Krieger, J. E.

AU - Kuramae, E. E.

AU - Laigret, F.

AU - Lambais, M. R.

AU - Leite, L. C.C.

AU - Lemos, E. G.M.

AU - Lemos, M. V.F.

AU - Lopes, S. A.

AU - Lopes, C. R.

AU - Machado, J. A.

AU - Machado, M. A.

AU - Madeira, A. M.B.N.

AU - Madeira, H. M.F.

AU - Marino, C. L.

AU - Marques, M. V.

AU - Martins, E. A.L.

AU - Martins, E. M.F.

AU - Matsukuma, A. Y.

AU - Menck, C. F.M.

AU - Miracca, E. C.

AU - Miyaki, C. Y.

AU - Monteiro-Vitorello, C. B.

AU - Moon, D. H.

AU - Nagai, M. A.

AU - Nascimento, A. L.T.O.

AU - Netto, L. E.S.

AU - Nhani, A.

AU - Nobrega, F. G.

AU - Nunes, L. R.

AU - Oliveira, M. A.

AU - de Oliveira, M. C.

AU - de Oliveira, R. C.

AU - Palmieri, D. A.

AU - Paris, A.

AU - Peixoto, B. R.

AU - Pereira, G. A.G.

AU - Pereira, H. A.

AU - Pesquero, J. B.

AU - Quaggio, R. B.

AU - Roberto, P. G.

AU - Rodrigues, V.

AU - Rosa, A. J.M.

AU - de Rosa, V. E.

AU - de Sá, R. G.

AU - Santelli, R. V.

AU - Sawasaki, H. E.

AU - da Silva, A. C.R.

AU - da Silva, A. M.

AU - da Silva, F. R.

AU - Silva, W. A.

AU - da Silveira, J. F.

AU - Silvestri, M. L.Z.

AU - Siqueira, W. J.

AU - de Souza, A. A.

AU - de Souza, A. P.

AU - Terenzi, M. F.

AU - Truffi, D.

AU - Tsai, S. M.

AU - Tsuhako, M. H.

AU - Vallada, H.

AU - Van Sluys, M. A.

AU - Verjovski-Almeida, S.

AU - Vettore, A. L.

AU - Zago, M. A.

AU - Zatz, M.

AU - Meidanis, J.

AU - Setubal, J. C.

PY - 2000/7/13

Y1 - 2000/7/13

N2 - Xylella fastidiosa is a fastidious, xylem-limited bacterium that causes a range of economically important plant diseases. Here we report the complete genome sequence of X. fastidiosa clone 9a5c, which causes citrus variegated chlorosis - a serious disease of orange trees. The genome comprises a 52.7% GC-rich 2,679,305-base-pair (bp) circular chromosome and 'two plasmids of 51,158 bp and 1,285 bp. We can assign putative functions to47% of the 2,904 predicted coding regions. Efficient metabolic functions are predicted, with sugars as the principal energy and carbon source, supporting existence in the nutrient-poor xylem sap. The mechanisms associated with pathogenicity and virulence involve toxins, antibiotics and ion sequestration systems, as well as bacterium-bacterium and bacterium-host interactions mediated by a range of proteins. Orthologues of some of these proteins have only been identified in animal and human pathogens; their presence in X. fastidiosa indicates that the molecular basis for bacterial pathogenicity is both conserved and independent of host. At least 83 genes are bacteriophage-derived and include virulence-associated genes from other bacteria, providing direct evidence of phage-mediated horizontal gene transfer.

AB - Xylella fastidiosa is a fastidious, xylem-limited bacterium that causes a range of economically important plant diseases. Here we report the complete genome sequence of X. fastidiosa clone 9a5c, which causes citrus variegated chlorosis - a serious disease of orange trees. The genome comprises a 52.7% GC-rich 2,679,305-base-pair (bp) circular chromosome and 'two plasmids of 51,158 bp and 1,285 bp. We can assign putative functions to47% of the 2,904 predicted coding regions. Efficient metabolic functions are predicted, with sugars as the principal energy and carbon source, supporting existence in the nutrient-poor xylem sap. The mechanisms associated with pathogenicity and virulence involve toxins, antibiotics and ion sequestration systems, as well as bacterium-bacterium and bacterium-host interactions mediated by a range of proteins. Orthologues of some of these proteins have only been identified in animal and human pathogens; their presence in X. fastidiosa indicates that the molecular basis for bacterial pathogenicity is both conserved and independent of host. At least 83 genes are bacteriophage-derived and include virulence-associated genes from other bacteria, providing direct evidence of phage-mediated horizontal gene transfer.

UR - http://www.scopus.com/inward/record.url?scp=0034644159&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034644159&partnerID=8YFLogxK

U2 - 10.1038/35018003

DO - 10.1038/35018003

M3 - Review article

C2 - 10910347

AN - SCOPUS:0034644159

SN - 0028-0836

VL - 406

SP - 151

EP - 157

JO - Nature

JF - Nature

IS - 6792

ER -

The genome sequence of the plant pathogen Xylella fastidiosa: The Xylella fastidiosa consortium of the organization for nucleotide sequencing and analysis, Sao Paulo, Brazil

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