Abstract
There is an ongoing need for more effective and less costly bone substitutes. It has previously been proposed that silica-containing bioactive glass would be more effective as a bone repair material because of its physiochemical properties. Three newly synthesized silica-containing bioactive glass formulations, HA-31 (25%), HA-11 (50%), and HA-13 (75%), were tested as biocompatible substrates for the continued proliferation and phenotype expression of human bone cells in vitro. Two currently available bioactive glasses (BioGlass®, Hydroxyapatite) served as comparisons. The biocompatibility of these bioglasses, as well as their osteoconductive properties, was assessed by employing primary cultures of human osteoblasts and human synoviocytes for 4 days. The results obtained demonstrated that the three new bioglasses enhanced the proliferative response of osteoblasts compared with osteoblasts cultured alone. Reverse Transcription Polymerase Chain Reaction (RT-PCR) analysis indicated that osteoblasts retained their phenotypic expression by continued expression of collagen type I and alkaline phosphatase. The newly synthesized preparations of silica-containing bioactive glass did not induce stimulation of proinflammatory markers iNOS and IL-1β in synoviocytes. In conclusion, the newly synthesized silica-containing bioactive glasses are biocompatible substrate for bone-forming osteoblasts. However, the formulations tested did not show significant advantage over the currently available bioactive glasses in vitro.
Original language | English (US) |
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Pages (from-to) | 1001-1008 |
Number of pages | 8 |
Journal | Journal of Biomedical Materials Research - Part A |
Volume | 67 |
Issue number | 3 |
DOIs | |
State | Published - Dec 1 2003 |
Externally published | Yes |
Keywords
- Bioactive glass
- Bone substitutes
- Calcium-phosphate
- Human osteoblasts and synoviocytes
- Silica
ASJC Scopus subject areas
- Ceramics and Composites
- Biomaterials
- Biomedical Engineering
- Metals and Alloys