The effect of silica-containing calcium-phosphate particles on human osteoblasts in vitro

Phong V. Phan, Mark Grzanna, James Chu, Anna Polotsky, Ahmed El-Ghannam, David Van Heerden, David S. Hungerford, Carmelita G. Frondoza

Research output: Contribution to journalArticlepeer-review

43 Scopus citations


There is an ongoing need for more effective and less costly bone substitutes. It has previously been proposed that silica-containing bioactive glass would be more effective as a bone repair material because of its physiochemical properties. Three newly synthesized silica-containing bioactive glass formulations, HA-31 (25%), HA-11 (50%), and HA-13 (75%), were tested as biocompatible substrates for the continued proliferation and phenotype expression of human bone cells in vitro. Two currently available bioactive glasses (BioGlass®, Hydroxyapatite) served as comparisons. The biocompatibility of these bioglasses, as well as their osteoconductive properties, was assessed by employing primary cultures of human osteoblasts and human synoviocytes for 4 days. The results obtained demonstrated that the three new bioglasses enhanced the proliferative response of osteoblasts compared with osteoblasts cultured alone. Reverse Transcription Polymerase Chain Reaction (RT-PCR) analysis indicated that osteoblasts retained their phenotypic expression by continued expression of collagen type I and alkaline phosphatase. The newly synthesized preparations of silica-containing bioactive glass did not induce stimulation of proinflammatory markers iNOS and IL-1β in synoviocytes. In conclusion, the newly synthesized silica-containing bioactive glasses are biocompatible substrate for bone-forming osteoblasts. However, the formulations tested did not show significant advantage over the currently available bioactive glasses in vitro.

Original languageEnglish (US)
Pages (from-to)1001-1008
Number of pages8
JournalJournal of Biomedical Materials Research - Part A
Issue number3
StatePublished - Dec 1 2003
Externally publishedYes


  • Bioactive glass
  • Bone substitutes
  • Calcium-phosphate
  • Human osteoblasts and synoviocytes
  • Silica

ASJC Scopus subject areas

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys


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