TY - JOUR
T1 - The effect of carbon dioxide pneumoperitoneum on the healing colonic anastomosis in rats
AU - Durães, Leonardo de Castro
AU - Farias, Igor Eduardo Caetano
AU - de Morais, Pedro Henrique Alves
AU - de Oliveira, Paulo Gonçalves
AU - Cavalcanti Neto, Florêncio Figueiredo
AU - Lino Junior, Ruy Souza
AU - Feres, Omar
AU - de Sousa, João Batista
PY - 2013/9
Y1 - 2013/9
N2 - PURPOSE: To investigate the effects of pneumoperitoneum on colonic anastomosis healing. METHODS: Colonic anastomosis was performed in 120 rats divided into four groups: Group I - pneumoperitoneum before laparotomy, Group II - pneumoperitoneum after laparorrhaphy, Group III - pneumoperitoneum before laparotomy and after laparorrhaphy, Group IV - no pneumoperitoneum (control group). Pneumoperitoneum pressure was 5 mmHg. Animals were killed on the 3rd, 7th and 14th postoperative day. Hhistopathological features, anastomosis breaking strength, collagen histomorphometry and hydroxyproline concentration were assessed. RESULTS: Breaking strength between groups: (day 3, p = 0.165; day 7, p = 0.219; day 14, p = 0.539). Histopathology revealed that group II had, on day 7, less infiltration of mononuclear cells (p = 0.006), greater infiltration of polymorphonuclear cells (p = 0.001) and greater necrosis (p = 0.001); and on day 14, less fibrosis. Histomorphometry revealed a decrease in collagen in groups I and III (p < 0.001) on day 7 and an increase in groups I and II on day 14 (p < 0.001). Hydroxyproline concentration was similar for groups on days 3 (p = 0.152), 7 (p = 0.913) or 14 (p = 0.981). CONCLUSION: Carbon dioxide does not impair the healing of colonic anastomosis in rats.
AB - PURPOSE: To investigate the effects of pneumoperitoneum on colonic anastomosis healing. METHODS: Colonic anastomosis was performed in 120 rats divided into four groups: Group I - pneumoperitoneum before laparotomy, Group II - pneumoperitoneum after laparorrhaphy, Group III - pneumoperitoneum before laparotomy and after laparorrhaphy, Group IV - no pneumoperitoneum (control group). Pneumoperitoneum pressure was 5 mmHg. Animals were killed on the 3rd, 7th and 14th postoperative day. Hhistopathological features, anastomosis breaking strength, collagen histomorphometry and hydroxyproline concentration were assessed. RESULTS: Breaking strength between groups: (day 3, p = 0.165; day 7, p = 0.219; day 14, p = 0.539). Histopathology revealed that group II had, on day 7, less infiltration of mononuclear cells (p = 0.006), greater infiltration of polymorphonuclear cells (p = 0.001) and greater necrosis (p = 0.001); and on day 14, less fibrosis. Histomorphometry revealed a decrease in collagen in groups I and III (p < 0.001) on day 7 and an increase in groups I and II on day 14 (p < 0.001). Hydroxyproline concentration was similar for groups on days 3 (p = 0.152), 7 (p = 0.913) or 14 (p = 0.981). CONCLUSION: Carbon dioxide does not impair the healing of colonic anastomosis in rats.
KW - Anastomosis
KW - Artificial
KW - Carbon dioxide
KW - Colon
KW - Pneumoperitoneum
KW - Rats
KW - Surgical
KW - Wound healing
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U2 - 10.1590/S0102-86502013000900008
DO - 10.1590/S0102-86502013000900008
M3 - Article
C2 - 24000061
AN - SCOPUS:84883611891
SN - 0102-8650
VL - 28
SP - 670
EP - 677
JO - Acta cirurgica brasileira
JF - Acta cirurgica brasileira
IS - 9
ER -