TY - JOUR
T1 - The deadenylase components Not2p, Not3p, and Not5p promote mRNA decapping
AU - Alhusaini, Najwa
AU - Coller, Jeff
N1 - Funding Information:
We thank the members of the Coller and Baker laboratories for advice and input into this work. Furthermore, we thank Dr. Marv Wickens for the gift of the yeast ORF-AD library. This work was funded by National Institutes of Health grant GM080465 to J.C.
Publisher Copyright:
© 2016 Alhusaini and Coller.
PY - 2016/5
Y1 - 2016/5
N2 - Decay of mRNA is essential for the efficient regulation of gene expression. A major pathway of mRNA degradation is initiated by the shortening of the poly(A) tail via the CCR4/NOT deadenylase complex. Deadenylation is followed by removal of the 5′ cap (i.e., decapping) and then 5′ to 3′ exonucleolytic decay of the message body. The highly conserved CCR4/NOT deadenylase complex consists of the exonucleases CCR4 and POP2/CAF1, as well as a group of four or five (depending on organism) accessory factors of unknown function, i.e., the NOT proteins. In this study, we find that Saccharomyces cerevisiae Not2p, Not3p, and Not5p (close paralogs of each other) are involved in promoting mRNA decapping. Furthermore, we find that Not3p and Not5p bind to the decapping activator protein Pat1p. Together, these data implicate the deadenylase complex in coordinating the downstream decapping reaction via Not2p, Not3p, and Not5p. This suggests that the coupling of deadenylation with decapping is, in part, a direct consequence of coordinated assembly of decay factors.
AB - Decay of mRNA is essential for the efficient regulation of gene expression. A major pathway of mRNA degradation is initiated by the shortening of the poly(A) tail via the CCR4/NOT deadenylase complex. Deadenylation is followed by removal of the 5′ cap (i.e., decapping) and then 5′ to 3′ exonucleolytic decay of the message body. The highly conserved CCR4/NOT deadenylase complex consists of the exonucleases CCR4 and POP2/CAF1, as well as a group of four or five (depending on organism) accessory factors of unknown function, i.e., the NOT proteins. In this study, we find that Saccharomyces cerevisiae Not2p, Not3p, and Not5p (close paralogs of each other) are involved in promoting mRNA decapping. Furthermore, we find that Not3p and Not5p bind to the decapping activator protein Pat1p. Together, these data implicate the deadenylase complex in coordinating the downstream decapping reaction via Not2p, Not3p, and Not5p. This suggests that the coupling of deadenylation with decapping is, in part, a direct consequence of coordinated assembly of decay factors.
KW - Deadenylation
KW - Decapping
KW - mRNA decay
KW - mRNA stability
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U2 - 10.1261/rna.054742.115
DO - 10.1261/rna.054742.115
M3 - Article
C2 - 26952104
AN - SCOPUS:84964490729
SN - 1355-8382
VL - 22
SP - 709
EP - 721
JO - RNA
JF - RNA
IS - 5
ER -