TY - JOUR
T1 - The capability of nonlinear optical characteristics as a predictor for cellular uptake of nanoparticles and cell damage
AU - Ardakani, Ali Abbasian
AU - Ghader, Alireza
AU - Asgari, Hamid
AU - Keshavarz, Marzieh
AU - Tazehmahalleh, Fatemeh Ebrahimi
AU - Majles Ara, Mohammad Hosein
AU - Malekzadeh, Malekeh
AU - Ghaznavi, Habib
AU - Shakeri-Zadeh, Ali
N1 - Funding Information:
All supports received from the Ministry of Health & Medical Education ( MOHME ) of Iran are acknowledged. This study was also supported by Zahedan University of Medical Sciences (grant number 7970 ).
Funding Information:
All supports received from the Ministry of Health & Medical Education (MOHME) of Iran are acknowledged. This study was also supported by Zahedan University of Medical Sciences (grant number 7970).
Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2019/9/1
Y1 - 2019/9/1
N2 - Current methods for determining the cellular effects of a treatment modality need expensive materials and much time to provide a researcher with results. The aim of this study was to evaluate the potential of nonlinear optical characteristics of cancer cells using Z-scan technique to monitor the level of cellular uptake and cell damage caused by a nanotechnology based treatment modality. Two nanocomplexes were synthesized and characterized. The first one was made of alginate hydrogel co-loaded with cisplatin and gold nanoparticles (AuNPs) named as ACA nanocomplex. The second one, named as AA nanocomplex, was the same as ACA, but without cisplatin and this AA nanocomplex was considered as the control for ACA. Different groups of CT26 mouse colon cancer cell line received various treatments of cisplatin, ACA, and AA nanocomplexes and then the samples were prepared for Z-scan studies. The MTT assay was used to evaluate the cytotoxicity induced by different treatment modalities. Transmission electron microscopy (TEM) and inductively coupled plasma-mass spectrometry (ICP-MS) were used for qualitative and quantitative assessments of the level of AuNPs cellular uptake. The trend of nonlinear optical properties changes for treated cells was in agreement with MTT, TEM and ICP-MS results. Z-scan technique was able to successfully indicate the occurrence of cell damage. It was also capable to determine the intensity of cell damage induced by ACA nanocomplex in comparison to free cisplatin. Furthermore, Z-scan results showed that it was able to discriminate the differences of optical properties of the cells incubated with ACA nanocomplex for various incubation times. Nonlinear optical characteristics of a cell may be considered as a reliable indicator to predict the level of cellular effects induced by a nanotechnology based treatment modality. The protocol suggested in this article does not waste materials, not take much time to provide the results, and it is inexpensive technique.
AB - Current methods for determining the cellular effects of a treatment modality need expensive materials and much time to provide a researcher with results. The aim of this study was to evaluate the potential of nonlinear optical characteristics of cancer cells using Z-scan technique to monitor the level of cellular uptake and cell damage caused by a nanotechnology based treatment modality. Two nanocomplexes were synthesized and characterized. The first one was made of alginate hydrogel co-loaded with cisplatin and gold nanoparticles (AuNPs) named as ACA nanocomplex. The second one, named as AA nanocomplex, was the same as ACA, but without cisplatin and this AA nanocomplex was considered as the control for ACA. Different groups of CT26 mouse colon cancer cell line received various treatments of cisplatin, ACA, and AA nanocomplexes and then the samples were prepared for Z-scan studies. The MTT assay was used to evaluate the cytotoxicity induced by different treatment modalities. Transmission electron microscopy (TEM) and inductively coupled plasma-mass spectrometry (ICP-MS) were used for qualitative and quantitative assessments of the level of AuNPs cellular uptake. The trend of nonlinear optical properties changes for treated cells was in agreement with MTT, TEM and ICP-MS results. Z-scan technique was able to successfully indicate the occurrence of cell damage. It was also capable to determine the intensity of cell damage induced by ACA nanocomplex in comparison to free cisplatin. Furthermore, Z-scan results showed that it was able to discriminate the differences of optical properties of the cells incubated with ACA nanocomplex for various incubation times. Nonlinear optical characteristics of a cell may be considered as a reliable indicator to predict the level of cellular effects induced by a nanotechnology based treatment modality. The protocol suggested in this article does not waste materials, not take much time to provide the results, and it is inexpensive technique.
KW - Cell damage
KW - Nanotechnology
KW - Nonlinear optical characteristics
KW - Refractive index
KW - Z-Scan
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UR - http://www.scopus.com/inward/citedby.url?scp=85071714882&partnerID=8YFLogxK
U2 - 10.1016/j.pdpdt.2019.07.023
DO - 10.1016/j.pdpdt.2019.07.023
M3 - Article
C2 - 31362112
AN - SCOPUS:85071714882
SN - 1572-1000
VL - 27
SP - 442
EP - 448
JO - Photodiagnosis and photodynamic therapy
JF - Photodiagnosis and photodynamic therapy
ER -