The biosynthesis of cytoplasmatic RNA in cells of Ehrlich ascites carcinoma under anaerobic conditions

Cells of Ehrlich ascites carcinoma were incubated under aerobic and anaerobic conditions with ³H-uridine, and the amount and radioactivity of poly(A)-containing and poly(A)-non-containing fraction of cytoplasmatic RNA were determined. It is shown that RNA biosynthesis, as judged by the labeled precursor incorporation, is nearly similar to that under oxygen supply conditions. The specific radioactivity of poly(A)-containing fraction of cytoplasmatic RNA of cells incubated under anaerobic conditions was 2 times as much as that under aerobic ones.