The 5′-untranslated region sequence of a potential new genotype of bovine viral diarrhea virus

S. Harpin; S. Mehdy Elahi; E. Cornaglia; R. H. Yolken; Y. Elazhary

doi:10.1007/BF01322754

The 5′-untranslated region sequence of a potential new genotype of bovine viral diarrhea virus

S. Harpin, S. Mehdy Elahi, E. Cornaglia, R. H. Yolken, Y. Elazhary

School of Medicine

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

The 5′ untranslated region (UTR) of several bovine viral diarrhea virus (BVDV) isolates from the severe Quebec outbreak was amplified by polymerase chain reaction (PCR) and sequenced. Sequences revealed the loss, for the BVDV type II isolates, of an internal PstI restriction site, which is present in all known BVDV type 1 5′UTR sequences. A single restriction enzyme digestion (PstI) of an aliquot of PCR product allowed us to differentiate BVDV type I and BVDV type II.

Original language	English (US)
Pages (from-to)	1285-1290
Number of pages	6
Journal	Archives of Virology
Volume	140
Issue number	7
DOIs	https://doi.org/10.1007/BF01322754
State	Published - Jul 1995

ASJC Scopus subject areas

Virology

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abstract = "The 5′ untranslated region (UTR) of several bovine viral diarrhea virus (BVDV) isolates from the severe Quebec outbreak was amplified by polymerase chain reaction (PCR) and sequenced. Sequences revealed the loss, for the BVDV type II isolates, of an internal PstI restriction site, which is present in all known BVDV type 1 5′UTR sequences. A single restriction enzyme digestion (PstI) of an aliquot of PCR product allowed us to differentiate BVDV type I and BVDV type II.",

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year = "1995",

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AU - Harpin, S.

AU - Mehdy Elahi, S.

AU - Cornaglia, E.

AU - Yolken, R. H.

AU - Elazhary, Y.

PY - 1995/7

Y1 - 1995/7

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AB - The 5′ untranslated region (UTR) of several bovine viral diarrhea virus (BVDV) isolates from the severe Quebec outbreak was amplified by polymerase chain reaction (PCR) and sequenced. Sequences revealed the loss, for the BVDV type II isolates, of an internal PstI restriction site, which is present in all known BVDV type 1 5′UTR sequences. A single restriction enzyme digestion (PstI) of an aliquot of PCR product allowed us to differentiate BVDV type I and BVDV type II.

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The 5′-untranslated region sequence of a potential new genotype of bovine viral diarrhea virus

Abstract

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