Tetramer staining for the detection of HLA-specific B cells

Donna P. Lucas; Mary S. Leffell; Andrea A. Zachary

doi:10.1007/978-1-62703-493-7_17

Tetramer staining for the detection of HLA-specific B cells

Donna P. Lucas, Mary S. Leffell, Andrea A. Zachary

School of Medicine

Research output: Chapter in Book/Report/Conference proceeding › Chapter

6 Scopus citations

Abstract

HLA-specific B cells can be identified, quantified, and isolated after staining with HLA tetramers. Quantification of these B cells can in turn identify individuals who are sensitized to HLA antigens and the isolation of these cells facilitates a variety of experimental investigations.

Original language	English (US)
Title of host publication	Transplantation Immunology
Subtitle of host publication	Methods and Protocols
Publisher	Humana Press Inc.
Pages	313-318
Number of pages	6
ISBN (Print)	9781627034920
DOIs	https://doi.org/10.1007/978-1-62703-493-7_17
State	Published - 2013

Publication series

Name	Methods in Molecular Biology
Volume	1034
ISSN (Print)	1064-3745

Keywords

B lymphocytes
HLA antibodies
HLA sensitization
HLA specific B Lymphocytes
HLA tetramers

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1007/978-1-62703-493-7_17

Cite this

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title = "Tetramer staining for the detection of HLA-specific B cells",

abstract = "HLA-specific B cells can be identified, quantified, and isolated after staining with HLA tetramers. Quantification of these B cells can in turn identify individuals who are sensitized to HLA antigens and the isolation of these cells facilitates a variety of experimental investigations.",

keywords = "B lymphocytes, HLA antibodies, HLA sensitization, HLA specific B Lymphocytes, HLA tetramers",

author = "Lucas, {Donna P.} and Leffell, {Mary S.} and Zachary, {Andrea A.}",

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AU - Lucas, Donna P.

AU - Leffell, Mary S.

AU - Zachary, Andrea A.

PY - 2013

Y1 - 2013

N2 - HLA-specific B cells can be identified, quantified, and isolated after staining with HLA tetramers. Quantification of these B cells can in turn identify individuals who are sensitized to HLA antigens and the isolation of these cells facilitates a variety of experimental investigations.

AB - HLA-specific B cells can be identified, quantified, and isolated after staining with HLA tetramers. Quantification of these B cells can in turn identify individuals who are sensitized to HLA antigens and the isolation of these cells facilitates a variety of experimental investigations.

KW - B lymphocytes

KW - HLA antibodies

KW - HLA sensitization

KW - HLA specific B Lymphocytes

KW - HLA tetramers

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M3 - Chapter

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AN - SCOPUS:84883216497

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T3 - Methods in Molecular Biology

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BT - Transplantation Immunology

PB - Humana Press Inc.

ER -

Tetramer staining for the detection of HLA-specific B cells

Abstract

Publication series

Keywords

ASJC Scopus subject areas

Access to Document

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