Tetra-fluorinated aromatic azide for highly efficient bioconjugation in living cells

Xuekang Cai; Dan Wang; Yasi Gao; Long Yi; Xing Yang; Zhen Xi

doi:10.1039/C8RA09303B

Tetra-fluorinated aromatic azide for highly efficient bioconjugation in living cells

Xuekang Cai, Dan Wang, Yasi Gao, Long Yi, Xing Yang, Zhen Xi

School of Medicine

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

We developed a fast strain-promoted azide-alkyne cycloaddition reaction (SPAAC) by tetra-fluorinated aromatic azide with a kinetic constant of 3.60 M ⁻¹ s ⁻¹ , which is among the fastest SPAAC ligations reported so far. We successfully employed the reaction for covalent labelling of proteins with high efficiency and for bioimaging of mitochondria in living cells. The reaction could be a generally useful toolbox for chemical biology and biomaterials.

Original language	English (US)
Pages (from-to)	23-26
Number of pages	4
Journal	RSC Advances
Volume	9
Issue number	1
DOIs	https://doi.org/10.1039/C8RA09303B
State	Published - 2019

ASJC Scopus subject areas

Chemistry(all)
Chemical Engineering(all)

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title = "Tetra-fluorinated aromatic azide for highly efficient bioconjugation in living cells",

abstract = " We developed a fast strain-promoted azide-alkyne cycloaddition reaction (SPAAC) by tetra-fluorinated aromatic azide with a kinetic constant of 3.60 M −1 s −1 , which is among the fastest SPAAC ligations reported so far. We successfully employed the reaction for covalent labelling of proteins with high efficiency and for bioimaging of mitochondria in living cells. The reaction could be a generally useful toolbox for chemical biology and biomaterials.",

author = "Xuekang Cai and Dan Wang and Yasi Gao and Long Yi and Xing Yang and Zhen Xi",

note = "Funding Information: This work was supported by the MOST (2017YFD0200900), NSFC (21572019, 21877008), 111 project (B14004), National Thousand Young Talents Program. Publisher Copyright: {\textcopyright} The Royal Society of Chemistry.",

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doi = "10.1039/C8RA09303B",

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T1 - Tetra-fluorinated aromatic azide for highly efficient bioconjugation in living cells

AU - Cai, Xuekang

AU - Wang, Dan

AU - Gao, Yasi

AU - Yi, Long

AU - Yang, Xing

AU - Xi, Zhen

N1 - Funding Information: This work was supported by the MOST (2017YFD0200900), NSFC (21572019, 21877008), 111 project (B14004), National Thousand Young Talents Program. Publisher Copyright: © The Royal Society of Chemistry.

PY - 2019

Y1 - 2019

N2 - We developed a fast strain-promoted azide-alkyne cycloaddition reaction (SPAAC) by tetra-fluorinated aromatic azide with a kinetic constant of 3.60 M −1 s −1 , which is among the fastest SPAAC ligations reported so far. We successfully employed the reaction for covalent labelling of proteins with high efficiency and for bioimaging of mitochondria in living cells. The reaction could be a generally useful toolbox for chemical biology and biomaterials.

AB - We developed a fast strain-promoted azide-alkyne cycloaddition reaction (SPAAC) by tetra-fluorinated aromatic azide with a kinetic constant of 3.60 M −1 s −1 , which is among the fastest SPAAC ligations reported so far. We successfully employed the reaction for covalent labelling of proteins with high efficiency and for bioimaging of mitochondria in living cells. The reaction could be a generally useful toolbox for chemical biology and biomaterials.

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Tetra-fluorinated aromatic azide for highly efficient bioconjugation in living cells

Abstract

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