TY - JOUR
T1 - Temporal and spatial regulation of phosphoinositide signaling mediates cytokinesis
AU - Janetopoulos, Chris
AU - Borleis, Jane
AU - Vazquez, Francisca
AU - Iijima, Miho
AU - Devreotes, Peter
N1 - Funding Information:
The authors wish to thank Dr. D. Robinson for providing the GFP-myosin II expression vector and for helpful discussions and critical reading of the manuscript. They also thank Dr. R. Firtel for providing the pi3k 1-2− cells. This work was supported by National Institutes of Health grants GM28007, GM34933, and GM071920 to P.D. and Department of Defense grant DAM 17-03-1-0195 to F.V. Sequence data for D . discoideum was obtained from the dictyBase website at http://www.dictybase.org/ .
PY - 2005/4
Y1 - 2005/4
N2 - Polarity is a prominent feature of both chemotaxis and cytokinesis. In chemotaxis, polarity is established by local accumulation of PI(3,4,5)P3 at the cell's leading edge, achieved through temporal and spatial regulation of PI3 kinases and the tumor suppressor, PTEN. We find that as migrating D. discoideum cells round up to enter cytokinesis, PI(3,4,5)P3 signaling is uniformly suppressed. Then, as the spindle and cell elongate, PI3 kinases and PTEN move to and function at the poles and furrow, respectively. Cell lines lacking both of these enzymatic activities fail to modulate PI(3,4,5)P3 levels, are defective in cytokinesis, and cannot divide in suspension. The cells continue to grow and duplicate their nuclei, generating large multinucleate cells. Furrows that fail to ingress between nuclei are unable to stably accumulate myosin filaments or suppress actin-filled ruffles. We propose that phosphoinositide-linked circuits, similar to those that bring about asymmetry during cell migration, also regulate polarity in cytokinesis.
AB - Polarity is a prominent feature of both chemotaxis and cytokinesis. In chemotaxis, polarity is established by local accumulation of PI(3,4,5)P3 at the cell's leading edge, achieved through temporal and spatial regulation of PI3 kinases and the tumor suppressor, PTEN. We find that as migrating D. discoideum cells round up to enter cytokinesis, PI(3,4,5)P3 signaling is uniformly suppressed. Then, as the spindle and cell elongate, PI3 kinases and PTEN move to and function at the poles and furrow, respectively. Cell lines lacking both of these enzymatic activities fail to modulate PI(3,4,5)P3 levels, are defective in cytokinesis, and cannot divide in suspension. The cells continue to grow and duplicate their nuclei, generating large multinucleate cells. Furrows that fail to ingress between nuclei are unable to stably accumulate myosin filaments or suppress actin-filled ruffles. We propose that phosphoinositide-linked circuits, similar to those that bring about asymmetry during cell migration, also regulate polarity in cytokinesis.
UR - https://www.scopus.com/pages/publications/16244377763
UR - https://www.scopus.com/pages/publications/16244377763#tab=citedBy
U2 - 10.1016/j.devcel.2005.02.010
DO - 10.1016/j.devcel.2005.02.010
M3 - Article
C2 - 15809030
AN - SCOPUS:16244377763
SN - 1534-5807
VL - 8
SP - 467
EP - 477
JO - Developmental Cell
JF - Developmental Cell
IS - 4
ER -