TY - JOUR
T1 - Temperature and species-dependent regulation of browning in retrobulbar fat
AU - Rajaii, Fatemeh
AU - Kim, Dong Won
AU - Pan, Jianbo
AU - Mahoney, Nicholas R.
AU - Eberhart, Charles G.
AU - Qian, Jiang
AU - Blackshaw, Seth
N1 - Funding Information:
We would like to thank Subhash Kulkarni for sharing the Wnt1:Cre mouse line. We would also like to thank Susan Aja and Michael Wolfgang for assistance with housing mice at thermoneutral temperature, Transcriptomics and Deep Sequencing Core (Johns Hopkins) for the preparation and sequencing of RNA-Seq libraries, and microscope facility (Johns Hopkins MICFAC, supported by the Award Number S10OD016374). FR is supported by the National Eye Institute of the National Institutes of Health under Award Number K08EY027093 and a Research to Prevent Blindness Unrestricted Grant. SB is supported by NIH Grant R01EY020560. DWK is supported by the Maryland Stem Cell Research Fund (2019-MSCRFF-5124).
Publisher Copyright:
© 2021, The Author(s).
PY - 2021/12
Y1 - 2021/12
N2 - Retrobulbar fat deposits surround the posterior retina and optic nerve head, but their function and origin are obscure. We report that mouse retrobulbar fat is a neural crest-derived tissue histologically and transcriptionally resembles interscapular brown fat. In contrast, human retrobulbar fat closely resembles white adipose tissue. Retrobulbar fat is also brown in other rodents, which are typically housed at temperatures below thermoneutrality, but is white in larger animals. We show that retrobulbar fat in mice housed at thermoneutral temperature show reduced expression of the brown fat marker Ucp1, and histological properties intermediate between white and brown fat. We conclude that retrobulbar fat can potentially serve as a site of active thermogenesis, that this capability is both temperature and species-dependent, and that this may facilitate regulation of intraocular temperature.
AB - Retrobulbar fat deposits surround the posterior retina and optic nerve head, but their function and origin are obscure. We report that mouse retrobulbar fat is a neural crest-derived tissue histologically and transcriptionally resembles interscapular brown fat. In contrast, human retrobulbar fat closely resembles white adipose tissue. Retrobulbar fat is also brown in other rodents, which are typically housed at temperatures below thermoneutrality, but is white in larger animals. We show that retrobulbar fat in mice housed at thermoneutral temperature show reduced expression of the brown fat marker Ucp1, and histological properties intermediate between white and brown fat. We conclude that retrobulbar fat can potentially serve as a site of active thermogenesis, that this capability is both temperature and species-dependent, and that this may facilitate regulation of intraocular temperature.
UR - http://www.scopus.com/inward/record.url?scp=85100491629&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85100491629&partnerID=8YFLogxK
U2 - 10.1038/s41598-021-82672-9
DO - 10.1038/s41598-021-82672-9
M3 - Article
C2 - 33542375
AN - SCOPUS:85100491629
SN - 2045-2322
VL - 11
JO - Scientific reports
JF - Scientific reports
IS - 1
M1 - 3094
ER -