Telomerase activity in germline and embryonic cells of Xenopus

L. L. Mantell; C. W. Greider

doi:10.1002/j.1460-2075.1994.tb06620.x

Telomerase activity in germline and embryonic cells of Xenopus

L. L. Mantell, C. W. Greider

Research output: Contribution to journal › Article › peer-review

116 Scopus citations

Abstract

Telomerase is a ribonucleoprotein which synthesizes telomere repeats onto chromosome ends. Telomerase activity is involved in telomere length maintenance. We used Xenopus laevis as a model system to study the expression of telomerase activity in germline cells and during early development. We identified a nonprocessive telomerase activity in manually dissected nuclei of Xenopus stage VI oocytes. Telomerase activity was detected throughout oogenesis and embryogenesis. Telomerase was active in both S and M phase cell cycle extracts, suggesting that telomerase activity is not regulated with chromosomal DNA replication.

Original language	English (US)
Pages (from-to)	3211-3217
Number of pages	7
Journal	EMBO Journal
Volume	13
Issue number	13
DOIs	https://doi.org/10.1002/j.1460-2075.1994.tb06620.x
State	Published - 1994
Externally published	Yes

Keywords

Cell cycle
Embryogenesis
Germline
Oogenesis
Telomerase

ASJC Scopus subject areas

General Neuroscience
Molecular Biology
General Biochemistry, Genetics and Molecular Biology
General Immunology and Microbiology

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10.1002/j.1460-2075.1994.tb06620.x

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AU - Greider, C. W.

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AB - Telomerase is a ribonucleoprotein which synthesizes telomere repeats onto chromosome ends. Telomerase activity is involved in telomere length maintenance. We used Xenopus laevis as a model system to study the expression of telomerase activity in germline cells and during early development. We identified a nonprocessive telomerase activity in manually dissected nuclei of Xenopus stage VI oocytes. Telomerase activity was detected throughout oogenesis and embryogenesis. Telomerase was active in both S and M phase cell cycle extracts, suggesting that telomerase activity is not regulated with chromosomal DNA replication.

KW - Cell cycle

KW - Embryogenesis

KW - Germline

KW - Oogenesis

KW - Telomerase

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Telomerase activity in germline and embryonic cells of Xenopus

Abstract

Keywords

ASJC Scopus subject areas

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