Taenia solium and Taenia ovis: Stage-specific expression of the vaccine antigen genes, TSOL18, TSOL16, and homologues, in oncospheres

Charles G. Gauci; Manuela R. Verástegui; Robert H. Gilman; Marshall W. Lightowlers

doi:10.1016/j.exppara.2006.01.006

Taenia solium and Taenia ovis: Stage-specific expression of the vaccine antigen genes, TSOL18, TSOL16, and homologues, in oncospheres

Charles G. Gauci, Manuela R. Verástegui, Robert H. Gilman, Marshall W. Lightowlers

Bloomberg School of Public Health

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

Recombinant antigens that have been cloned from Taenia solium and Taenia ovis have been shown to be highly effective when used as vaccines against cysticercosis in the intermediate hosts. This study investigated the presence of mRNA encoding the TSOL18 and TSOL16 antigens in different life-cycle stages of T. solium, and their related homologues in T. ovis. Reverse transcription-PCR and Southern blotting demonstrated that the antigens are stage-specifically expressed in the oncosphere. The apparent absence of expression of TSOL18 in the metacestode life-cycle stage suggests that the vaccine based on this antigen targets exclusively the early stages in the development of the parasite.

Original language	English (US)
Pages (from-to)	272-275
Number of pages	4
Journal	Experimental Parasitology
Volume	113
Issue number	4
DOIs	https://doi.org/10.1016/j.exppara.2006.01.006
State	Published - Aug 2006

Keywords

Cestode
Cysticercosis
Echinococcus granulosus
Expression
Gene
Oncosphere
PCR
Parasite
RT-PCR
Taenia ovis
Taenia saginanta
Taenia solium
Vaccine
cDNA
complementary DNA
mRNA
messenger RNA
polymerase chain reaction
reverse transcription-PCR

ASJC Scopus subject areas

Parasitology
Immunology
Infectious Diseases

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10.1016/j.exppara.2006.01.006

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title = "Taenia solium and Taenia ovis: Stage-specific expression of the vaccine antigen genes, TSOL18, TSOL16, and homologues, in oncospheres",

abstract = "Recombinant antigens that have been cloned from Taenia solium and Taenia ovis have been shown to be highly effective when used as vaccines against cysticercosis in the intermediate hosts. This study investigated the presence of mRNA encoding the TSOL18 and TSOL16 antigens in different life-cycle stages of T. solium, and their related homologues in T. ovis. Reverse transcription-PCR and Southern blotting demonstrated that the antigens are stage-specifically expressed in the oncosphere. The apparent absence of expression of TSOL18 in the metacestode life-cycle stage suggests that the vaccine based on this antigen targets exclusively the early stages in the development of the parasite.",

keywords = "Cestode, Cysticercosis, Echinococcus granulosus, Expression, Gene, Oncosphere, PCR, Parasite, RT-PCR, Taenia ovis, Taenia saginanta, Taenia solium, Vaccine, cDNA, complementary DNA, mRNA, messenger RNA, polymerase chain reaction, reverse transcription-PCR",

author = "Gauci, {Charles G.} and Ver{\'a}stegui, {Manuela R.} and Gilman, {Robert H.} and Lightowlers, {Marshall W.}",

note = "Funding Information: This work was funded with research grants from the National Health and Medical Research Council of Australia and the Wellcome Trust. M.V. and R.G. acknowledge support from the Gates Foundation.",

year = "2006",

month = aug,

doi = "10.1016/j.exppara.2006.01.006",

language = "English (US)",

volume = "113",

pages = "272--275",

journal = "Experimental Parasitology",

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T2 - Stage-specific expression of the vaccine antigen genes, TSOL18, TSOL16, and homologues, in oncospheres

AU - Gauci, Charles G.

AU - Verástegui, Manuela R.

AU - Gilman, Robert H.

AU - Lightowlers, Marshall W.

N1 - Funding Information: This work was funded with research grants from the National Health and Medical Research Council of Australia and the Wellcome Trust. M.V. and R.G. acknowledge support from the Gates Foundation.

PY - 2006/8

Y1 - 2006/8

N2 - Recombinant antigens that have been cloned from Taenia solium and Taenia ovis have been shown to be highly effective when used as vaccines against cysticercosis in the intermediate hosts. This study investigated the presence of mRNA encoding the TSOL18 and TSOL16 antigens in different life-cycle stages of T. solium, and their related homologues in T. ovis. Reverse transcription-PCR and Southern blotting demonstrated that the antigens are stage-specifically expressed in the oncosphere. The apparent absence of expression of TSOL18 in the metacestode life-cycle stage suggests that the vaccine based on this antigen targets exclusively the early stages in the development of the parasite.

AB - Recombinant antigens that have been cloned from Taenia solium and Taenia ovis have been shown to be highly effective when used as vaccines against cysticercosis in the intermediate hosts. This study investigated the presence of mRNA encoding the TSOL18 and TSOL16 antigens in different life-cycle stages of T. solium, and their related homologues in T. ovis. Reverse transcription-PCR and Southern blotting demonstrated that the antigens are stage-specifically expressed in the oncosphere. The apparent absence of expression of TSOL18 in the metacestode life-cycle stage suggests that the vaccine based on this antigen targets exclusively the early stages in the development of the parasite.

KW - Cestode

KW - Cysticercosis

KW - Echinococcus granulosus

KW - Expression

KW - Gene

KW - Oncosphere

KW - PCR

KW - Parasite

KW - RT-PCR

KW - Taenia ovis

KW - Taenia saginanta

KW - Taenia solium

KW - Vaccine

KW - cDNA

KW - complementary DNA

KW - mRNA

KW - messenger RNA

KW - polymerase chain reaction

KW - reverse transcription-PCR

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M3 - Article

C2 - 16510142

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SN - 0014-4894

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JO - Experimental Parasitology

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IS - 4

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Taenia solium and Taenia ovis: Stage-specific expression of the vaccine antigen genes, TSOL18, TSOL16, and homologues, in oncospheres

Abstract

Keywords

ASJC Scopus subject areas

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