Systemic RNA Interference Deficiency-1 (SID-1) Extracellular Domain Selectively Binds Long Double-stranded RNA and Is Required for RNA Transport by SID-1

Weiqiang Li; Kristin S. Koutmou; Daniel J. Leahy; Min Li

doi:10.1074/jbc.M115.658864

Systemic RNA Interference Deficiency-1 (SID-1) Extracellular Domain Selectively Binds Long Double-stranded RNA and Is Required for RNA Transport by SID-1

Weiqiang Li, Kristin S. Koutmou, Daniel J. Leahy, Min Li

School of Medicine

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Background: SystemicRNAinterference deficiency-1 (SID-1) is a membrane protein required for cellular uptake ofRNAin C. elegans. Results: SID-1 extracellular domain selectively binds long dsRNA. Conclusion: Binding affinity between SID-1 ECD and dsRNA is related to RNA transport efficiency by SID-1. Significance: This defines SID-1 ECD as a functional domain for dsRNA recognition.

Original language	English (US)
Pages (from-to)	18904-18913
Number of pages	10
Journal	Journal of Biological Chemistry
Volume	290
Issue number	31
DOIs	https://doi.org/10.1074/jbc.M115.658864
State	Published - Jul 31 2015

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Cell Biology

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abstract = "Background: SystemicRNAinterference deficiency-1 (SID-1) is a membrane protein required for cellular uptake ofRNAin C. elegans. Results: SID-1 extracellular domain selectively binds long dsRNA. Conclusion: Binding affinity between SID-1 ECD and dsRNA is related to RNA transport efficiency by SID-1. Significance: This defines SID-1 ECD as a functional domain for dsRNA recognition.",

author = "Weiqiang Li and Koutmou, {Kristin S.} and Leahy, {Daniel J.} and Min Li",

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AU - Li, Weiqiang

AU - Koutmou, Kristin S.

AU - Leahy, Daniel J.

AU - Li, Min

PY - 2015/7/31

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AB - Background: SystemicRNAinterference deficiency-1 (SID-1) is a membrane protein required for cellular uptake ofRNAin C. elegans. Results: SID-1 extracellular domain selectively binds long dsRNA. Conclusion: Binding affinity between SID-1 ECD and dsRNA is related to RNA transport efficiency by SID-1. Significance: This defines SID-1 ECD as a functional domain for dsRNA recognition.

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Systemic RNA Interference Deficiency-1 (SID-1) Extracellular Domain Selectively Binds Long Double-stranded RNA and Is Required for RNA Transport by SID-1

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