TY - JOUR
T1 - Systemic Metabolite Changes in Wild-type C57BL/6 Mice Fed Black Raspberries
AU - Pan, Pan
AU - Skaer, Chad W.
AU - Wang, Hsin Tzu
AU - Kreiser, Michael A.
AU - Stirdivant, Steven M.
AU - Oshima, Kiyoko
AU - Huang, Yi Wen
AU - Young, Matthew R.
AU - Wang, Li Shu
N1 - Funding Information:
The authors thank Chieh-Ti Kuo for her assistance in this study. They also thank Dr. Gary D. Stoner and Dr. John F. Lechner for their time and effort of reviewing and editing this manuscript. Furthermore, they would like to thank Covance Inc. for their help on fatty acid profiling analysis of control diet and BRB powder. The authors thank the National Institutes of Health (grant 5 R01 CA148818) and American Cancer Society (RSG-13-138-01—CNE to L.-S. Wang) for funding this study.
Publisher Copyright:
© 2017 Taylor & Francis Group, LLC.
PY - 2017/2/17
Y1 - 2017/2/17
N2 - Introduction: Freeze-dried black raspberries (BRBs) elicit chemopreventive effects against colorectal cancer in humans and in rodents. The objective of this study was to investigate potential BRB-caused metabolite changes using wild-type (WT) C57BL/6 mice. Methods and results: WT mice were fed either control diet or control diet supplemented with 5% BRBs for 8 wk. A nontargeted metabolomic analysis was conducted on colonic mucosa, liver, and fecal specimens collected from both diet groups. BRBs significantly changed the levels of 41 colonic mucosa metabolites, 40 liver metabolites, and 34 fecal metabolites compared to control diet-fed mice. BRBs reduced 34 lipid metabolites in colonic mucosa and increased levels of amino acids in liver. One metabolite, 3-[3-(sulfooxy) phenyl] propanoic acid, might be a useful biomarker of BRB consumption. In addition, BRB powder was found to contain 30-fold higher levels of linolenate compared to control diets. Consistently, multiple omega-3 polyunsaturated fatty acids (ω-3 PUFAs), including stearidonate, docosapentaenoate (ω-3 DPA), eicosapentaenoate (EPA), and docosahexaenoate (DHA), were significantly elevated in livers of BRB-fed mice. Conclusion: The data from the current study suggest that BRBs produce systemic metabolite changes in multiple tissue matrices, supporting our hypothesis that BRBs may serve as both a chemopreventive agent and a beneficial dietary supplement.
AB - Introduction: Freeze-dried black raspberries (BRBs) elicit chemopreventive effects against colorectal cancer in humans and in rodents. The objective of this study was to investigate potential BRB-caused metabolite changes using wild-type (WT) C57BL/6 mice. Methods and results: WT mice were fed either control diet or control diet supplemented with 5% BRBs for 8 wk. A nontargeted metabolomic analysis was conducted on colonic mucosa, liver, and fecal specimens collected from both diet groups. BRBs significantly changed the levels of 41 colonic mucosa metabolites, 40 liver metabolites, and 34 fecal metabolites compared to control diet-fed mice. BRBs reduced 34 lipid metabolites in colonic mucosa and increased levels of amino acids in liver. One metabolite, 3-[3-(sulfooxy) phenyl] propanoic acid, might be a useful biomarker of BRB consumption. In addition, BRB powder was found to contain 30-fold higher levels of linolenate compared to control diets. Consistently, multiple omega-3 polyunsaturated fatty acids (ω-3 PUFAs), including stearidonate, docosapentaenoate (ω-3 DPA), eicosapentaenoate (EPA), and docosahexaenoate (DHA), were significantly elevated in livers of BRB-fed mice. Conclusion: The data from the current study suggest that BRBs produce systemic metabolite changes in multiple tissue matrices, supporting our hypothesis that BRBs may serve as both a chemopreventive agent and a beneficial dietary supplement.
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U2 - 10.1080/01635581.2017.1263748
DO - 10.1080/01635581.2017.1263748
M3 - Article
C2 - 28094560
AN - SCOPUS:85009826802
SN - 0163-5581
VL - 69
SP - 299
EP - 306
JO - Nutrition and Cancer
JF - Nutrition and Cancer
IS - 2
ER -