The systemic effects of parenterally administered purified cholera enterotoxin were studied in dogs. Single doses of 100 μg or more were frequently lethal, causing death after 2 to 10 days. The cause of death was not determined, but dogs did not develop diarrhea. Intravenous enterotoxin produced several biochemical abnormalities including: hyperglycemic, hyponatremia and increased levels of serum alkaline phosphatase, and glutamic-oxalacetic transaminase (SGOT). The hyperglycemia was delayed in onset and lasted at least 5 days in fasting dogs. Insulin release in response to hyperglycemia appeared normal. Serum alkaline phosphatase remained elevated for more than 5 days. The rise in serum alkaline phosphatase was due to an increase in alkaline phosphatase of liver origin, and liver alkaline phosphatase content was shown to increase more rapidly than that in serum. Hyponatremia and increased SGOT levels were also of several days' duration. A formalinized cholera toxoid produced none of the above effects. The significance of these observations is discussed in relation to the use of cholera enterotoxin or toxoid as an immunizing agent, in relation to the present understanding of the mechanism of action of cholera enterotoxin, and in relation to the possibility that absorbed enterotoxin is partly responsible for intestinal secretion in cholera.
|Number of pages
|The Journal of laboratory and clinical medicine
|Published - Jan 1972
ASJC Scopus subject areas
- Pathology and Forensic Medicine