Sintez i sekretsiia rekombinantnogo belka--produkta onkogena v-sis v kletkakh drozhzheǐ Saccharomyces cerevisiae.

E. A. Ratovitskiǐ; A. B. Rudenko; N. A. Vasinova; O. V. Kidgotko; B. K. Nisman

Sintez i sekretsiia rekombinantnogo belka--produkta onkogena v-sis v kletkakh drozhzheǐ Saccharomyces cerevisiae.

Translated title of the contribution: Synthesis and secretion of recombinant protein--the product of v-sis oncogene in Saccharomyces cerevisiae cells

E. A. Ratovitskiǐ, A. B. Rudenko, N. A. Vasinova, O. V. Kidgotko, B. K. Nisman

Research output: Contribution to journal › Article › peer-review

Abstract

The recombinant plasmid DNA YEp secl-v-sis was constructed. This plasmid was able to code for the synthesis and secretion into the cultural medium of the protein-product of oncogene v-sis. Transcription, copy number and stability of the plasmid DNA were studied under the conditions of galactose induction. The v-sis protein was determined by gel electrophoresis and immunoblotting methods and assayed for cell-proliferation activity in the mammalian cell culture.

Translated title of the contribution	Synthesis and secretion of recombinant protein--the product of v-sis oncogene in Saccharomyces cerevisiae cells
Original language	Russian
Pages (from-to)	20-24
Number of pages	5
Journal	Molekuliarnaia genetika, mikrobiologiia i virusologiia
Issue number	5
State	Published - May 1990
Externally published	Yes

ASJC Scopus subject areas

Medicine(all)

Cite this

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title = "Sintez i sekretsiia rekombinantnogo belka--produkta onkogena v-sis v kletkakh drozhzheǐ Saccharomyces cerevisiae.",

abstract = "The recombinant plasmid DNA YEp secl-v-sis was constructed. This plasmid was able to code for the synthesis and secretion into the cultural medium of the protein-product of oncogene v-sis. Transcription, copy number and stability of the plasmid DNA were studied under the conditions of galactose induction. The v-sis protein was determined by gel electrophoresis and immunoblotting methods and assayed for cell-proliferation activity in the mammalian cell culture.",

author = "Ratovitskiǐ, {E. A.} and Rudenko, {A. B.} and Vasinova, {N. A.} and Kidgotko, {O. V.} and Nisman, {B. K.}",

year = "1990",

month = may,

language = "Russian",

pages = "20--24",

journal = "Molekuliarnaia genetika, mikrobiologiia i virusologiia",

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AU - Ratovitskiǐ, E. A.

AU - Rudenko, A. B.

AU - Vasinova, N. A.

AU - Kidgotko, O. V.

AU - Nisman, B. K.

PY - 1990/5

Y1 - 1990/5

N2 - The recombinant plasmid DNA YEp secl-v-sis was constructed. This plasmid was able to code for the synthesis and secretion into the cultural medium of the protein-product of oncogene v-sis. Transcription, copy number and stability of the plasmid DNA were studied under the conditions of galactose induction. The v-sis protein was determined by gel electrophoresis and immunoblotting methods and assayed for cell-proliferation activity in the mammalian cell culture.

AB - The recombinant plasmid DNA YEp secl-v-sis was constructed. This plasmid was able to code for the synthesis and secretion into the cultural medium of the protein-product of oncogene v-sis. Transcription, copy number and stability of the plasmid DNA were studied under the conditions of galactose induction. The v-sis protein was determined by gel electrophoresis and immunoblotting methods and assayed for cell-proliferation activity in the mammalian cell culture.

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Sintez i sekretsiia rekombinantnogo belka--produkta onkogena v-sis v kletkakh drozhzheǐ Saccharomyces cerevisiae.

Abstract

ASJC Scopus subject areas

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