Synthesis and enantiomeric resolution of tritiated (D,L)‐3‐hydroxykynurenine

John R. Lever, Karen A. Canella, Clifford L. Eastman, Tomas R. Guilarte

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


The synthesis, purification and enantiomeric resolution of tritiated (D,L)‐3‐hydroxykynurenine are described. Bromination of racemic 3‐hydroxykynurenine, catalytic dehalotritiation and purification by ion pair, reverse phase HPLC afforded tritiated material of high radiochemical purity (99.8%) and moderate specific radioactivity (3.64 Ci/mmol). Enantiomerically pure (D)‐ and (L)‐stereoisomers were isolated using chiral HPLC conditions. The stability of the label toward isotopic exchange was assessed to be sufficient for biochemical studies.

Original languageEnglish (US)
Pages (from-to)1331-1339
Number of pages9
JournalJournal of Labelled Compounds and Radiopharmaceuticals
Issue number11
StatePublished - Nov 1990

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Radiology Nuclear Medicine and imaging
  • Drug Discovery
  • Spectroscopy
  • Organic Chemistry


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