Surface supported bilayer platform for studies of lateral association of proteins in membranes

Mikhail Merzlyakov; Edwin Li; Kalina Hristova

doi:10.1116/1.2912096

Surface supported bilayer platform for studies of lateral association of proteins in membranes

Mikhail Merzlyakov, Edwin Li, Kalina Hristova

Whiting School of Engineering

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Here, the authors review how surface supported bilayers can be engineered and how Förster resonance energy transfer (FRET) can be used to quantify interactions between transmembrane peptides in these bilayers. The requirements for the surface supported platform are (1) lateral mobility of the peptides, (2) transmembrane orientation of the peptides, and (3) capabilities for FRET measurements. To satisfy these requirements, a new assembly method, termed "directed assembly" was developed. This assembly method could have broad utility in basic studies of proteins in membranes and in biotechnological applications.

Original language	English (US)
Pages (from-to)	FA80-FA84
Journal	Biointerphases
Volume	3
Issue number	2
DOIs	https://doi.org/10.1116/1.2912096
State	Published - Jun 2008

ASJC Scopus subject areas

Chemistry(all)
Biomaterials
Materials Science(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)

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10.1116/1.2912096

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Surface supported bilayer platform for studies of lateral association of proteins in membranes

Abstract

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