@article{be689dd21e7c4beaad5c93a139ce4dbe,
title = "Surface-enhanced Raman scattering analysis of urine from deceased donors as a prognostic tool for kidney transplant outcome",
abstract = "We report the utility of surface-enhanced Raman scattering (SERS) analysis of urine from deceased donors for prognosis of kidney transplant outcomes. Iodide-modified silver nanoparticles were used as the enabler for sensitive measurements of urine proteins. Principal component analysis (PCA) and linear discriminant analysis (LDA) were employed for the statistical analysis of the SERS data. Thirty urine samples in three classes were analysed. The ATN class consists of donors whose kidneys had acute tubular necrosis (ATN), the most common type of acute kidney injury (AKI) with high risk of poor graft performance in recipients, yet yielded acceptable transplant outcome. The DGF class is comprised of donors whose kidney had delayed graft function (DGF) in recipients. The control class includes donors whose kidneys did not have donor ATN or recipient DGF. We show a sensitivity of more than 90 % in differentiating the ATN class from the DGF and control classes. Our methodology can thus help clinicians choose kidneys in the high-risk ATN category for transplant which would otherwise be discarded. Our research is impactful in that it could serve as a valuable guidance to expand the deceased donor pool to include those perceived as high-risk AKI type based on common urinary biomarkers. Picutre: Scheme of SERS analysis of urine samples from deceased donors for kidney transplant outcome indication.",
keywords = "SERS, acute tubular necrosis (ATN), deceased donor, delayed graft function (DGF), kidney transplant, optical diagnosis, principal component analysis (PCA), urine",
author = "Jingmao Chi and Yiwei Ma and Weng, {Francis L.} and Heather Thiessen-Philbrook and Parikh, {Chirag R.} and Henry Du",
note = "Funding Information: The data reported here have been supplied by the United Network for Organ Sharing (UNOS) as the contractor for the Organ Procurement and Transplantation Network (OPTN). The interpretation and reporting of these data are the responsibility of the author(s) and in no way should be seen as an official policy of or interpretation by the OPTN or the U.S. Government. We are tremendously grateful for the study participation of partners at four organ procurement organizations: Gift of Life Philadelphia (Rick Hasz, Sharon West, Vicky Reilly), the New York Organ Donor Network (Harvey Lerner, Anthony Guidice, Allison Hoffman), the Michigan Organ and Tissue Donation Program (Burton Mattice, Susan Shay), and the New Jersey Sharing Network (William Reitsma, Cindy Godfrey, Alene Steward, Joel Padilla Benitez). Study Investigators: Peter P Reese, Renal-Electrolyte and Hypertension Division, Perelman School of Medicine, Department of Biostatistics and Epidemiology, and Leonard Davis Institute of Health Economics, University of Utah, Salt Lake City, UT, USA; Bernd Schr{\"o}ppel Section of Nephrology, University Hospital, Ulm, Germany; Mona D. Doshi, Wayne State University, Detroit, Michigan. This work was supported by (i) Stevens Ignition Grant Initiative, (ii) the National Institutes of Health grant R01DK-93770 and grant K24DK090203, (iii) a Roche Organ Transplantation Research Foundation Award to Dr. Parikh, and (iv) the Health Resources and Services Administration contract 234–2005-37011C. Funding Information: Acknowledgements The data reported here have been supplied by the United Network for Organ Sharing (UNOS) as the contractor for the Organ Procurement and Transplantation Network (OPTN). The interpretation and reporting of these data are the responsibility of the author (s) and in no way should be seen as an official policy of or interpretation by the OPTN or the U.S. Government. We are tremendously grateful for the study participation of partners at four organ procurement organizations: Gift of Life Philadelphia (Rick Hasz, Sharon West, Vicky Reilly), the New York Organ Donor Network (Harvey Lerner, Anthony Guidice, Allison Hoffman), the Michigan Organ and Tissue Donation Program (Burton Mattice, Susan Shay), and the New Jersey Sharing Network (William Re-itsma, Cindy Godfrey, Alene Steward, Joel Padilla Beni-tez). Study Investigators: Peter P Reese, Renal-Electrolyte and Hypertension Division, Perelman School of Medicine, Department of Biostatistics and Epidemiology, and Leonard Davis Institute of Health Economics, University of Utah, Salt Lake City, UT, USA; Bernd Schr{\"o}ppel Section of Nephrology, University Hospital, Ulm, Germany; Mona D. Doshi, Wayne State University, Detroit, Michigan. This work was supported by (i) Stevens Ignition Grant Initiative, (ii) the National Institutes of Health grant R01DK-93770 and grant K24DK090203, (iii) a Roche Organ Transplantation Research Foundation Award to Dr. Parikh, and (iv) the Health Resources and Services Administration contract 234–2005-37011C. Publisher Copyright: {\textcopyright} 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim",
year = "2017",
month = dec,
doi = "10.1002/jbio.201700019",
language = "English (US)",
volume = "10",
pages = "1743--1755",
journal = "Journal of Biophotonics",
issn = "1864-063X",
publisher = "Wiley-VCH Verlag",
number = "12",
}