TY - JOUR
T1 - Suppression of Tumorigenicity of a Human Lung Carcinoma Line by Nontumorigenic Bronchial Epithelial Cells in Somatic Cell Hybrids
AU - Kaighn, M. Edward
AU - Iman, Deborah S.
AU - Pauls, Elisa A.
AU - Harris, Curtis C.
AU - Gabrielson, Edward W.
PY - 1990/3/15
Y1 - 1990/3/15
N2 - Hybrid cell lines between HuT292-DM, a human lung carcinoma line resistant to 6-thioguanine and ouabain, and either normal human bronchial epithelial cells (NHBE) or an SV40 “immortalized”1 but nontumorigenic derivative thereof (BEAS-2B), have been isolated by double selection. Hybrids of NHBE and HuT292-DM cells senesced after 40–43 population doublings in culture. In contrast, hybrids of BEAS-2B and HuT292-DM showed no sign of a culture “crisis” and have an indefinite life span. HuT292-DM cells produced tumors in 100% of athymic nude mice with a mean latency of 27 days, whereas tumorigenicity was totally suppressed in 76% of the BEAS-2B x HuT292-DM hybrids, with a 2-to 3-fold increased tumor latency in the remaining 24% of these hybrids. While the hybrids are hypotriploid to hypotetraploid, the parental lines are hypodiploid. The growth of HuT292-DM cells is stimulated, whereas NHBE and BEAS-2B cells are inhibited by serum. The growth response of the BEAS-2B x HuT292-DM hybrids to serum is similar to that of HuT292-DM ceUs. Thus, tumorigenicity and culture longevity are dom-inantly controlled by the nontumorigenic parent (NHBE or BEAS-2B). On the other hand, serum responsiveness is more similar to that of the tumorigenic parent (HuT292-DM).
AB - Hybrid cell lines between HuT292-DM, a human lung carcinoma line resistant to 6-thioguanine and ouabain, and either normal human bronchial epithelial cells (NHBE) or an SV40 “immortalized”1 but nontumorigenic derivative thereof (BEAS-2B), have been isolated by double selection. Hybrids of NHBE and HuT292-DM cells senesced after 40–43 population doublings in culture. In contrast, hybrids of BEAS-2B and HuT292-DM showed no sign of a culture “crisis” and have an indefinite life span. HuT292-DM cells produced tumors in 100% of athymic nude mice with a mean latency of 27 days, whereas tumorigenicity was totally suppressed in 76% of the BEAS-2B x HuT292-DM hybrids, with a 2-to 3-fold increased tumor latency in the remaining 24% of these hybrids. While the hybrids are hypotriploid to hypotetraploid, the parental lines are hypodiploid. The growth of HuT292-DM cells is stimulated, whereas NHBE and BEAS-2B cells are inhibited by serum. The growth response of the BEAS-2B x HuT292-DM hybrids to serum is similar to that of HuT292-DM ceUs. Thus, tumorigenicity and culture longevity are dom-inantly controlled by the nontumorigenic parent (NHBE or BEAS-2B). On the other hand, serum responsiveness is more similar to that of the tumorigenic parent (HuT292-DM).
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M3 - Article
C2 - 2306741
AN - SCOPUS:0025271095
SN - 0008-5472
VL - 50
SP - 1890
EP - 1896
JO - Cancer Research
JF - Cancer Research
IS - 6
ER -