Supercharged green fluorescent proteins as bimodal reporter genes for CEST MRI and optical imaging

Amnon Bar-Shir; Yajie Liang; Kannie W.Y. Chan; Assaf A. Gilad; Jeff W.M. Bulte

doi:10.1039/c4cc10195b

Supercharged green fluorescent proteins as bimodal reporter genes for CEST MRI and optical imaging

Amnon Bar-Shir, Yajie Liang, Kannie W.Y. Chan, Assaf A. Gilad, Jeff W.M. Bulte

School of Medicine

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Superpositively charged mutants of green fluorescent protein (GFP) demonstrated a dramatically improved chemical exchange saturation transfer (CEST) MRI contrast compared to their wild type counterparts. The mutants +36 GFP and +48 GFP were successfully expressed in mammalian cells and retained part of their fluorescence, making them a new potential bimodal reporter gene.

Original language	English (US)
Pages (from-to)	4869-4871
Number of pages	3
Journal	Chemical Communications
Volume	51
Issue number	23
DOIs	https://doi.org/10.1039/c4cc10195b
State	Published - Mar 21 2015

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Chemistry(all)
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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abstract = "Superpositively charged mutants of green fluorescent protein (GFP) demonstrated a dramatically improved chemical exchange saturation transfer (CEST) MRI contrast compared to their wild type counterparts. The mutants +36 GFP and +48 GFP were successfully expressed in mammalian cells and retained part of their fluorescence, making them a new potential bimodal reporter gene.",

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AU - Gilad, Assaf A.

AU - Bulte, Jeff W.M.

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AB - Superpositively charged mutants of green fluorescent protein (GFP) demonstrated a dramatically improved chemical exchange saturation transfer (CEST) MRI contrast compared to their wild type counterparts. The mutants +36 GFP and +48 GFP were successfully expressed in mammalian cells and retained part of their fluorescence, making them a new potential bimodal reporter gene.

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Supercharged green fluorescent proteins as bimodal reporter genes for CEST MRI and optical imaging

Abstract

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