[3H]3,4-Methylenedioxymethamphetamine (MDMA) interactions with brain membranes and glass fiber filter paper

Samuel S. Wang; George A. Ricaurte; Stephen J. Peroutka

doi:10.1016/0014-2999(87)90485-7

[³H]3,4-Methylenedioxymethamphetamine (MDMA) interactions with brain membranes and glass fiber filter paper

Samuel S. Wang, George A. Ricaurte, Stephen J. Peroutka

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

The binding of [³H]3,4-methylenedioxymethamphetamine ([³H]MDMA) to both rat brain membrane preparations and glass fiber filter papers was analyzed in the present study. Saturation studies indicate that [³H]MDMA binding is saturable and monophasic in both the presence and absence of rat brain membranes. This apparent 'specific' binding of [³H]MDMA in both the presence and absence of brain homogenates was totally eliminated by pretreating glass fiber filter papers with polyethylenimine. Drug competition studies demonstrated that [³H]MDMA binding displays a distinct 'pharmacological' profile in the absence of brain tissue. These data indicate that apparent [³H]MDMA binding to rat brain homogenates results from artifactual of [³H]MDMA to glass fiber filter paper. In addition, uptake of [³H]MDMA into rat brain synaptosomes could not be detected. We conclude the [³H]MDMA has limited usefulness in radioligand binding studies.

Original language	English (US)
Pages (from-to)	439-443
Number of pages	5
Journal	European Journal of Pharmacology
Volume	138
Issue number	3
DOIs	https://doi.org/10.1016/0014-2999(87)90485-7
State	Published - Jun 26 1987
Externally published	Yes

Keywords

(Artifactual binding)
3,4-Methylenedioxymethamphetamine (MDMA)
Polyethylenimine
Radioligand binding studies

ASJC Scopus subject areas

Pharmacology

Access to Document

10.1016/0014-2999(87)90485-7

Cite this

@article{9c404cd321bf46efba09a4d0e6473eb0,

title = "[3H]3,4-Methylenedioxymethamphetamine (MDMA) interactions with brain membranes and glass fiber filter paper",

abstract = "The binding of [3H]3,4-methylenedioxymethamphetamine ([3H]MDMA) to both rat brain membrane preparations and glass fiber filter papers was analyzed in the present study. Saturation studies indicate that [3H]MDMA binding is saturable and monophasic in both the presence and absence of rat brain membranes. This apparent 'specific' binding of [3H]MDMA in both the presence and absence of brain homogenates was totally eliminated by pretreating glass fiber filter papers with polyethylenimine. Drug competition studies demonstrated that [3H]MDMA binding displays a distinct 'pharmacological' profile in the absence of brain tissue. These data indicate that apparent [3H]MDMA binding to rat brain homogenates results from artifactual of [3H]MDMA to glass fiber filter paper. In addition, uptake of [3H]MDMA into rat brain synaptosomes could not be detected. We conclude the [3H]MDMA has limited usefulness in radioligand binding studies.",

keywords = "(Artifactual binding), 3,4-Methylenedioxymethamphetamine (MDMA), Polyethylenimine, Radioligand binding studies",

author = "Wang, {Samuel S.} and Ricaurte, {George A.} and Peroutka, {Stephen J.}",

note = "Funding Information: We thank Faith Smith for assistance in manuscript preparation. This work was supported in part by the John A. and George U Hartford Foundation, the McKnight Foundation and NIH Grants NS 12151-12 and NS 23560-01.",

year = "1987",

month = jun,

day = "26",

doi = "10.1016/0014-2999(87)90485-7",

language = "English (US)",

volume = "138",

pages = "439--443",

journal = "European Journal of Pharmacology",

issn = "0014-2999",

publisher = "Elsevier",

number = "3",

}

TY - JOUR

T1 - [3H]3,4-Methylenedioxymethamphetamine (MDMA) interactions with brain membranes and glass fiber filter paper

AU - Wang, Samuel S.

AU - Ricaurte, George A.

AU - Peroutka, Stephen J.

N1 - Funding Information: We thank Faith Smith for assistance in manuscript preparation. This work was supported in part by the John A. and George U Hartford Foundation, the McKnight Foundation and NIH Grants NS 12151-12 and NS 23560-01.

PY - 1987/6/26

Y1 - 1987/6/26

N2 - The binding of [3H]3,4-methylenedioxymethamphetamine ([3H]MDMA) to both rat brain membrane preparations and glass fiber filter papers was analyzed in the present study. Saturation studies indicate that [3H]MDMA binding is saturable and monophasic in both the presence and absence of rat brain membranes. This apparent 'specific' binding of [3H]MDMA in both the presence and absence of brain homogenates was totally eliminated by pretreating glass fiber filter papers with polyethylenimine. Drug competition studies demonstrated that [3H]MDMA binding displays a distinct 'pharmacological' profile in the absence of brain tissue. These data indicate that apparent [3H]MDMA binding to rat brain homogenates results from artifactual of [3H]MDMA to glass fiber filter paper. In addition, uptake of [3H]MDMA into rat brain synaptosomes could not be detected. We conclude the [3H]MDMA has limited usefulness in radioligand binding studies.

AB - The binding of [3H]3,4-methylenedioxymethamphetamine ([3H]MDMA) to both rat brain membrane preparations and glass fiber filter papers was analyzed in the present study. Saturation studies indicate that [3H]MDMA binding is saturable and monophasic in both the presence and absence of rat brain membranes. This apparent 'specific' binding of [3H]MDMA in both the presence and absence of brain homogenates was totally eliminated by pretreating glass fiber filter papers with polyethylenimine. Drug competition studies demonstrated that [3H]MDMA binding displays a distinct 'pharmacological' profile in the absence of brain tissue. These data indicate that apparent [3H]MDMA binding to rat brain homogenates results from artifactual of [3H]MDMA to glass fiber filter paper. In addition, uptake of [3H]MDMA into rat brain synaptosomes could not be detected. We conclude the [3H]MDMA has limited usefulness in radioligand binding studies.

KW - (Artifactual binding)

KW - 3,4-Methylenedioxymethamphetamine (MDMA)

KW - Polyethylenimine

KW - Radioligand binding studies

UR - http://www.scopus.com/inward/record.url?scp=0023665740&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023665740&partnerID=8YFLogxK

U2 - 10.1016/0014-2999(87)90485-7

DO - 10.1016/0014-2999(87)90485-7

M3 - Article

C2 - 2887443

AN - SCOPUS:0023665740

SN - 0014-2999

VL - 138

SP - 439

EP - 443

JO - European Journal of Pharmacology

JF - European Journal of Pharmacology

IS - 3

ER -