31P NMR of mammalian cells encapsulated in alginate gels utilizing a new phosphate‐free perfusion medium

K. Shankar Narayan; E. A. Moress; J. C. Chatham; P. B. Barker

doi:10.1002/nbm.1940030104

³¹P NMR of mammalian cells encapsulated in alginate gels utilizing a new phosphate‐free perfusion medium

K. Shankar Narayan, E. A. Moress, J. C. Chatham, P. B. Barker

School of Medicine

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

A simple technique permitting long term ³¹P magnetic resonance spectroscopy of cultured mammalian cells is described. Cells are encapsulated in calcium alginate gels and perfused with a new phosphate‐free medium designed for maintenance of high cell viability. Intracellular pH and β‐NTP/P_i ratios, studied in three cell types, remained stable over a 16–20 h NMR monitoring period. The techniques described may be useful for monitoring cell metabolism with a variety of cell types.

Original language	English (US)
Pages (from-to)	23-26
Number of pages	4
Journal	NMR in biomedicine
Volume	3
Issue number	1
DOIs	https://doi.org/10.1002/nbm.1940030104
State	Published - Feb 1990

ASJC Scopus subject areas

Molecular Medicine
Radiology Nuclear Medicine and imaging
Spectroscopy

Access to Document

10.1002/nbm.1940030104

Cite this

@article{7f0f74b70f6a4d4795f072df61df8dcd,

title = "31P NMR of mammalian cells encapsulated in alginate gels utilizing a new phosphate‐free perfusion medium",

abstract = "A simple technique permitting long term 31P magnetic resonance spectroscopy of cultured mammalian cells is described. Cells are encapsulated in calcium alginate gels and perfused with a new phosphate‐free medium designed for maintenance of high cell viability. Intracellular pH and β‐NTP/Pi ratios, studied in three cell types, remained stable over a 16–20 h NMR monitoring period. The techniques described may be useful for monitoring cell metabolism with a variety of cell types.",

author = "Narayan, {K. Shankar} and Moress, {E. A.} and Chatham, {J. C.} and Barker, {P. B.}",

year = "1990",

month = feb,

doi = "10.1002/nbm.1940030104",

language = "English (US)",

volume = "3",

pages = "23--26",

journal = "NMR in biomedicine",

issn = "0952-3480",

publisher = "John Wiley and Sons Ltd",

number = "1",

}

TY - JOUR

T1 - 31P NMR of mammalian cells encapsulated in alginate gels utilizing a new phosphate‐free perfusion medium

AU - Narayan, K. Shankar

AU - Moress, E. A.

AU - Chatham, J. C.

AU - Barker, P. B.

PY - 1990/2

Y1 - 1990/2

N2 - A simple technique permitting long term 31P magnetic resonance spectroscopy of cultured mammalian cells is described. Cells are encapsulated in calcium alginate gels and perfused with a new phosphate‐free medium designed for maintenance of high cell viability. Intracellular pH and β‐NTP/Pi ratios, studied in three cell types, remained stable over a 16–20 h NMR monitoring period. The techniques described may be useful for monitoring cell metabolism with a variety of cell types.

AB - A simple technique permitting long term 31P magnetic resonance spectroscopy of cultured mammalian cells is described. Cells are encapsulated in calcium alginate gels and perfused with a new phosphate‐free medium designed for maintenance of high cell viability. Intracellular pH and β‐NTP/Pi ratios, studied in three cell types, remained stable over a 16–20 h NMR monitoring period. The techniques described may be useful for monitoring cell metabolism with a variety of cell types.

UR - http://www.scopus.com/inward/record.url?scp=0025377869&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025377869&partnerID=8YFLogxK

U2 - 10.1002/nbm.1940030104

DO - 10.1002/nbm.1940030104

M3 - Article

C2 - 2390450

AN - SCOPUS:0025377869

SN - 0952-3480

VL - 3

SP - 23

EP - 26

JO - NMR in biomedicine

JF - NMR in biomedicine

IS - 1

ER -

³¹P NMR of mammalian cells encapsulated in alginate gels utilizing a new phosphate‐free perfusion medium

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this