@article{0dd3e597f0d14692b1f6f35f7ed574d3,
title = "Sumoylation promotes optimal apc/c activation and timely anaphase",
abstract = "The Anaphase Promoting Complex/Cyclosome (APC/C) is a ubiquitin E3 ligase that functions as the gatekeeper to mitotic exit. APC/C activity is controlled by an interplay of multiple pathways during mitosis, including the spindle assembly checkpoint (SAC), that are not yet fully understood. Here, we show that sumoylation of the APC4 subunit of the APC/C peaks during mitosis and is critical for timely APC/C activation and anaphase onset. We have also identified a functionally important SUMO interacting motif in the cullin-homology domain of APC2 located near the APC4 sumoylation sites and APC/C catalytic core. Our findings provide evidence of an important regulatory role for SUMO modification and binding in affecting APC/C activation and mitotic exit.",
author = "Lee, {Christine C.} and Bing Li and Hongtao Yu and Matunis, {Michael J.}",
note = "Funding Information: We thank Andrew Holland for YFP-H2B HeLa cells and thoughtful discussions and assistance; Mary Dasso for the 6xHis-SUMO2 U2OS cells and thoughtful discussions and suggestions; Janice Evans for the use of the microscope for timelapse imaging; all of the members of the Matunis Lab and Marie Morrow for their discussions, ideas, and critical reading of the manuscript. We would also like to thank Dr Alfred CO Vergetaal for sharing unpublished data. Work in the Yu laboratory is supported by grants from the Cancer Prevention and Research Institute of Texas (RP120717-P2 and RP160667-P2) and the Welch Foundation (I-1441). HY is an Investigator with the Howard Hughes Medical Institute. This work was funded by National Institutes of Health Grant R01 GM060980 (to MJM) and T32 CA009110 (to CL). Publisher Copyright: {\textcopyright} Lee et al.",
year = "2018",
month = mar,
day = "8",
doi = "10.7554/eLife.29539",
language = "English (US)",
volume = "7",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications",
}