Abstract
Genes whose expression changes with administration of abused substances provide candidate biochemical mechanisms for drug-induced long-term brain changes. To identify such genes, and to avoid the false positive results frequently obtained from differential display PCR, we applied a subtracted differential display (SDD) approach. We subtracted single-stranded cDNA prepared from drug-treated animals with excess mRNA from saline-treated animals, and visa versa, prior to differential display amplifications. Two of the initial amphetamine-regulated cDNAs identified in this fashion encoded calcineurin A, a neuron-specific protein phosphatase catalytic subunit whose striatal expression was upregulated ca. 1.5-fold. SDD may enhance the utility of differential display approaches to identifying regulated genes in tissues in which mRNA complexities are high.
Original language | English (US) |
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Pages (from-to) | 344-347 |
Number of pages | 4 |
Journal | Molecular Brain Research |
Volume | 53 |
Issue number | 1-2 |
DOIs | |
State | Published - Jan 1998 |
Keywords
- Amphetamine
- Drug abuse
- Gene regulation
- PCR differential display
- Subtraction
ASJC Scopus subject areas
- Molecular Biology
- Cellular and Molecular Neuroscience