Subclass distribution of human IgG autoantibodies in pemphigus

Christian C. Jones; Robert G. Hamilton; Robert E. Jordon

doi:10.1007/BF00915155

Subclass distribution of human IgG autoantibodies in pemphigus

Christian C. Jones, Robert G. Hamilton, Robert E. Jordon

Research output: Contribution to journal › Article › peer-review

80 Scopus citations

Abstract

The distribution of IgG subclasses in the intercellular substance (ICS) reactive autoantibodies in serum of 10 patients with pemphigus was analyzed by semiquantitative indirect immunofluorescence (IF) using the HP series of monoclonal antibodies specific for the four human IgG subclasses. IgG4 ICS specific autoantibody was present in all 10 sera at a titer of 10 to 320, while IgG1 antibodies were found in 9 of 10 sera at a seemingly lower level. IgG3 autoantibodies were detected in the serum of one patient, only after isolation of IgG using ion-exchange chromatography. Autoantibodies of IgG subclass 2 were not detectable in any of the 10 sera tested. One of the ten patients displayed circulating anti-ICS antibodies of only the IgG4 isotype.

Original language	English (US)
Pages (from-to)	43-49
Number of pages	7
Journal	Journal of Clinical Immunology
Volume	8
Issue number	1
DOIs	https://doi.org/10.1007/BF00915155
State	Published - Jan 1988
Externally published	Yes

Keywords

Pemphigus
enzyme-linked immunosorbent assay (ELISA)
human IgG subclasses
immunofluorescence
intercellular substance autoantibodies

ASJC Scopus subject areas

Immunology and Allergy
Immunology

Access to Document

10.1007/BF00915155

Cite this

@article{430bcce99a8347fdbe81c7c169df36cd,

title = "Subclass distribution of human IgG autoantibodies in pemphigus",

abstract = "The distribution of IgG subclasses in the intercellular substance (ICS) reactive autoantibodies in serum of 10 patients with pemphigus was analyzed by semiquantitative indirect immunofluorescence (IF) using the HP series of monoclonal antibodies specific for the four human IgG subclasses. IgG4 ICS specific autoantibody was present in all 10 sera at a titer of 10 to 320, while IgG1 antibodies were found in 9 of 10 sera at a seemingly lower level. IgG3 autoantibodies were detected in the serum of one patient, only after isolation of IgG using ion-exchange chromatography. Autoantibodies of IgG subclass 2 were not detectable in any of the 10 sera tested. One of the ten patients displayed circulating anti-ICS antibodies of only the IgG4 isotype.",

keywords = "Pemphigus, enzyme-linked immunosorbent assay (ELISA), human IgG subclasses, immunofluorescence, intercellular substance autoantibodies",

author = "Jones, {Christian C.} and Hamilton, {Robert G.} and Jordon, {Robert E.}",

year = "1988",

month = jan,

doi = "10.1007/BF00915155",

language = "English (US)",

volume = "8",

pages = "43--49",

journal = "Journal of Clinical Immunology",

issn = "0271-9142",

publisher = "Springer New York",

number = "1",

}

TY - JOUR

T1 - Subclass distribution of human IgG autoantibodies in pemphigus

AU - Jones, Christian C.

AU - Hamilton, Robert G.

AU - Jordon, Robert E.

PY - 1988/1

Y1 - 1988/1

N2 - The distribution of IgG subclasses in the intercellular substance (ICS) reactive autoantibodies in serum of 10 patients with pemphigus was analyzed by semiquantitative indirect immunofluorescence (IF) using the HP series of monoclonal antibodies specific for the four human IgG subclasses. IgG4 ICS specific autoantibody was present in all 10 sera at a titer of 10 to 320, while IgG1 antibodies were found in 9 of 10 sera at a seemingly lower level. IgG3 autoantibodies were detected in the serum of one patient, only after isolation of IgG using ion-exchange chromatography. Autoantibodies of IgG subclass 2 were not detectable in any of the 10 sera tested. One of the ten patients displayed circulating anti-ICS antibodies of only the IgG4 isotype.

AB - The distribution of IgG subclasses in the intercellular substance (ICS) reactive autoantibodies in serum of 10 patients with pemphigus was analyzed by semiquantitative indirect immunofluorescence (IF) using the HP series of monoclonal antibodies specific for the four human IgG subclasses. IgG4 ICS specific autoantibody was present in all 10 sera at a titer of 10 to 320, while IgG1 antibodies were found in 9 of 10 sera at a seemingly lower level. IgG3 autoantibodies were detected in the serum of one patient, only after isolation of IgG using ion-exchange chromatography. Autoantibodies of IgG subclass 2 were not detectable in any of the 10 sera tested. One of the ten patients displayed circulating anti-ICS antibodies of only the IgG4 isotype.

KW - Pemphigus

KW - enzyme-linked immunosorbent assay (ELISA)

KW - human IgG subclasses

KW - immunofluorescence

KW - intercellular substance autoantibodies

UR - http://www.scopus.com/inward/record.url?scp=0023870865&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023870865&partnerID=8YFLogxK

U2 - 10.1007/BF00915155

DO - 10.1007/BF00915155

M3 - Article

C2 - 3284896

AN - SCOPUS:0023870865

SN - 0271-9142

VL - 8

SP - 43

EP - 49

JO - Journal of Clinical Immunology

JF - Journal of Clinical Immunology

IS - 1

ER -

Subclass distribution of human IgG autoantibodies in pemphigus

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this