@inbook{035c8deb0ab84de980a45d87cae47795,
title = "Subcellular optogenetic stimulation platform for studying activity-dependent axon myelination in vitro",
abstract = "Activity-dependent myelination modulates neuron conduction velocity and as such it is essential for a correct wiring of a whole nervous system. Increasing myelination through inducing neuron activity has been proposed as a treatment strategy for demyelination diseases. Yet, the mechanisms and the effects of activity-dependent myelination remain elusive—new tools are needed. In this chapter, we describe a novel compartmentalized device integrated with an optogenetic stimulator for studying activity-dependent myelination in vitro. The platform can be modified to include multiple cell types, stimulation modes, and experimental readouts to answer a specific research question. This versatility combined with a precise control over spatial extent of the stimulation and the stimulation pattern make the proposed platform a valuable tool for molecular myelination studies.",
keywords = "Activity-dependent myelination, Compartmentalized culture, In vitro myelination, Microfluidics, Neurons, Oligodendrocytes, Optogenetics, Subcellular stimulation",
author = "Agata Blasiak and Sudip Nag and Yang, {In Hong}",
note = "Publisher Copyright: {\textcopyright} 2018, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2018",
doi = "10.1007/978-1-4939-7862-5_16",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "207--224",
booktitle = "Methods in Molecular Biology",
}