Studies of Simian virus 40 DNA. VII. A cleavage map of the SV40 genome

Kathleen J. Danna; George H. Sack; Daniel Nathans

doi:10.1016/0022-2836(73)90122-8

Studies of Simian virus 40 DNA. VII. A cleavage map of the SV40 genome

Kathleen J. Danna, George H. Sack, Daniel Nathans

School of Medicine

Research output: Contribution to journal › Article › peer-review

236 Scopus citations

Abstract

A physical map of the Simian virus 40 genome has been constructed on the basis of specific cleavage of Simian virus 40 DNA by bacterial restriction endonucleases. The 11 fragments produced by enzyme from Hemophilus influenzae have been ordered by analysis of partial digest products and by analysis of an overlapping set of fragments produced by enzyme from Hemophilus parainfluenzae. In addition, the single site in SV40 DNA cleaved by the Escherichia coli R_I restriction endonuclease has been located. With this site as a reference point, the H. influenzae cleavage sites and the H. parainfluenzae cleavage sites have been localized on the map.

Original language	English (US)
Pages (from-to)	363-368,IN9-IN10,369-376
Journal	Journal of molecular biology
Volume	78
Issue number	2
DOIs	https://doi.org/10.1016/0022-2836(73)90122-8
State	Published - Aug 5 1973

ASJC Scopus subject areas

Structural Biology
Molecular Biology

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10.1016/0022-2836(73)90122-8

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title = "Studies of Simian virus 40 DNA. VII. A cleavage map of the SV40 genome",

abstract = "A physical map of the Simian virus 40 genome has been constructed on the basis of specific cleavage of Simian virus 40 DNA by bacterial restriction endonucleases. The 11 fragments produced by enzyme from Hemophilus influenzae have been ordered by analysis of partial digest products and by analysis of an overlapping set of fragments produced by enzyme from Hemophilus parainfluenzae. In addition, the single site in SV40 DNA cleaved by the Escherichia coli RI restriction endonuclease has been located. With this site as a reference point, the H. influenzae cleavage sites and the H. parainfluenzae cleavage sites have been localized on the map.",

author = "Danna, {Kathleen J.} and Sack, {George H.} and Daniel Nathans",

note = "Funding Information: We thank Dr Theresa Lee for her help in some of the experiments reported. This research was supported by grants from the National Cancer Institute, United States Public Health Service, and the Whitehall Foundation, Inc. One of us (G. H. S.) was supported by training grant no. GM00624 from the United States Public Health Service and another author (K. J. D.) was a predoctoral fellow of the United States Public Health Service. The results reported in this paper were presented at the Cold Spring Harbor Symposium on Tumor Viruses in August, 1972.",

year = "1973",

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doi = "10.1016/0022-2836(73)90122-8",

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AU - Danna, Kathleen J.

AU - Sack, George H.

AU - Nathans, Daniel

N1 - Funding Information: We thank Dr Theresa Lee for her help in some of the experiments reported. This research was supported by grants from the National Cancer Institute, United States Public Health Service, and the Whitehall Foundation, Inc. One of us (G. H. S.) was supported by training grant no. GM00624 from the United States Public Health Service and another author (K. J. D.) was a predoctoral fellow of the United States Public Health Service. The results reported in this paper were presented at the Cold Spring Harbor Symposium on Tumor Viruses in August, 1972.

PY - 1973/8/5

Y1 - 1973/8/5

N2 - A physical map of the Simian virus 40 genome has been constructed on the basis of specific cleavage of Simian virus 40 DNA by bacterial restriction endonucleases. The 11 fragments produced by enzyme from Hemophilus influenzae have been ordered by analysis of partial digest products and by analysis of an overlapping set of fragments produced by enzyme from Hemophilus parainfluenzae. In addition, the single site in SV40 DNA cleaved by the Escherichia coli RI restriction endonuclease has been located. With this site as a reference point, the H. influenzae cleavage sites and the H. parainfluenzae cleavage sites have been localized on the map.

AB - A physical map of the Simian virus 40 genome has been constructed on the basis of specific cleavage of Simian virus 40 DNA by bacterial restriction endonucleases. The 11 fragments produced by enzyme from Hemophilus influenzae have been ordered by analysis of partial digest products and by analysis of an overlapping set of fragments produced by enzyme from Hemophilus parainfluenzae. In addition, the single site in SV40 DNA cleaved by the Escherichia coli RI restriction endonuclease has been located. With this site as a reference point, the H. influenzae cleavage sites and the H. parainfluenzae cleavage sites have been localized on the map.

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Studies of Simian virus 40 DNA. VII. A cleavage map of the SV40 genome

Abstract

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