Structure and mechanism of DNA topoisomerase II

James M. Berger; Steven J. Gamblin; Stephen C. Harrison; James C. Wang

doi:10.1038/379225a0

Structure and mechanism of DNA topoisomerase II

James M. Berger, Steven J. Gamblin, Stephen C. Harrison, James C. Wang

Research output: Contribution to journal › Article › peer-review

713 Scopus citations

Abstract

The crystal structure of a large fragment of yeast type II DNA topoisomerase reveals a heart-shaped dimeric protein with a large central hole. It provides a molecular model of the enzyme as an ATP-modulated clamp with two sets of jaws at opposite ends, connected by multiple joints. An enzyme with bound DNA can admit a second DNA duplex through one set of jaws, transport it through the cleaved first duplex, and expel it through the other set of jaws.

Original language	English (US)
Pages (from-to)	225-232
Number of pages	8
Journal	Nature
Volume	379
Issue number	6562
DOIs	https://doi.org/10.1038/379225a0
State	Published - Jan 18 1996
Externally published	Yes

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title = "Structure and mechanism of DNA topoisomerase II",

abstract = "The crystal structure of a large fragment of yeast type II DNA topoisomerase reveals a heart-shaped dimeric protein with a large central hole. It provides a molecular model of the enzyme as an ATP-modulated clamp with two sets of jaws at opposite ends, connected by multiple joints. An enzyme with bound DNA can admit a second DNA duplex through one set of jaws, transport it through the cleaved first duplex, and expel it through the other set of jaws.",

author = "Berger, {James M.} and Gamblin, {Steven J.} and Harrison, {Stephen C.} and Wang, {James C.}",

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AU - Gamblin, Steven J.

AU - Harrison, Stephen C.

AU - Wang, James C.

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AB - The crystal structure of a large fragment of yeast type II DNA topoisomerase reveals a heart-shaped dimeric protein with a large central hole. It provides a molecular model of the enzyme as an ATP-modulated clamp with two sets of jaws at opposite ends, connected by multiple joints. An enzyme with bound DNA can admit a second DNA duplex through one set of jaws, transport it through the cleaved first duplex, and expel it through the other set of jaws.

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Structure and mechanism of DNA topoisomerase II

Abstract

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