Structural similarities between topoisomerases that cleave one or both DNA strands

James M. Berger; Deborah Fass; James C. Wang; Stephen C. Harrison

doi:10.1073/pnas.95.14.7876

Structural similarities between topoisomerases that cleave one or both DNA strands

James M. Berger, Deborah Fass, James C. Wang, Stephen C. Harrison

Research output: Contribution to journal › Article › peer-review

87 Scopus citations

Abstract

Type IA and type II DNA topoisomerases are distinguished by their ability to cleave one or two strands, respectively, of a DNA duplex. Both types have been proposed to use an 'enzyme-bridging' mechanism, in which a break is formed in a DNA strand and a gap is opened between the broken pieces to allow passage of a second DNA strand or duplex segment. Although the type IA and type II topoisomerase structures appear overall quite different from one another, unexpected similarities between several structural elements suggest that members of the two subfamilies may use comparable mechanisms to bind and cleave DNA.

Original language	English (US)
Pages (from-to)	7876-7881
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	95
Issue number	14
DOIs	https://doi.org/10.1073/pnas.95.14.7876
State	Published - Jul 7 1998
Externally published	Yes

Keywords

Catabolite-activator protein-like domain
DNA binding
DNA cleavage
Rossmann fold

ASJC Scopus subject areas

General

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abstract = "Type IA and type II DNA topoisomerases are distinguished by their ability to cleave one or two strands, respectively, of a DNA duplex. Both types have been proposed to use an 'enzyme-bridging' mechanism, in which a break is formed in a DNA strand and a gap is opened between the broken pieces to allow passage of a second DNA strand or duplex segment. Although the type IA and type II topoisomerase structures appear overall quite different from one another, unexpected similarities between several structural elements suggest that members of the two subfamilies may use comparable mechanisms to bind and cleave DNA.",

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T1 - Structural similarities between topoisomerases that cleave one or both DNA strands

AU - Berger, James M.

AU - Fass, Deborah

AU - Wang, James C.

AU - Harrison, Stephen C.

PY - 1998/7/7

Y1 - 1998/7/7

N2 - Type IA and type II DNA topoisomerases are distinguished by their ability to cleave one or two strands, respectively, of a DNA duplex. Both types have been proposed to use an 'enzyme-bridging' mechanism, in which a break is formed in a DNA strand and a gap is opened between the broken pieces to allow passage of a second DNA strand or duplex segment. Although the type IA and type II topoisomerase structures appear overall quite different from one another, unexpected similarities between several structural elements suggest that members of the two subfamilies may use comparable mechanisms to bind and cleave DNA.

AB - Type IA and type II DNA topoisomerases are distinguished by their ability to cleave one or two strands, respectively, of a DNA duplex. Both types have been proposed to use an 'enzyme-bridging' mechanism, in which a break is formed in a DNA strand and a gap is opened between the broken pieces to allow passage of a second DNA strand or duplex segment. Although the type IA and type II topoisomerase structures appear overall quite different from one another, unexpected similarities between several structural elements suggest that members of the two subfamilies may use comparable mechanisms to bind and cleave DNA.

KW - Catabolite-activator protein-like domain

KW - DNA binding

KW - DNA cleavage

KW - Rossmann fold

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Structural similarities between topoisomerases that cleave one or both DNA strands

Abstract

Keywords

ASJC Scopus subject areas

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