Structural dissection of ATP turnover in the prototypical GHL ATPase TopoVI

Kevin D. Corbett, James M. Berger

Research output: Contribution to journalArticlepeer-review

55 Scopus citations


GHL proteins are functionally diverse enzymes defined by the presence of a conserved ATPase domain that self-associates to trap substrate upon nucleotide binding. The structural states adopted by these enzymes during nucleotide hydrolysis and product release, and their consequences for enzyme catalysis, have remained unclear. Here, we have determined a complete structural map of the ATP turnover cycle for topoVI-B, the ATPase subunit of the archaeal GHL enzyme topoisomerase VI. With this ensemble of structures, we show that significant conformational changes in the subunit occur first upon ATP binding, and subsequently upon release of hydrolyzed Pi. Together, these data provide a structural framework for understanding the role of ATP hydrolysis in the type II topoisomerase reaction. Our results also suggest that the GHL ATPase module is a molecular switch in which ATP hydrolysis serves as a prerequisite but not a driving force for substrate-dependent structural transitions in the enzyme.

Original languageEnglish (US)
Pages (from-to)873-882
Number of pages10
Issue number6
StatePublished - Jun 2005
Externally publishedYes

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology


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