@article{27189449bfbd49e39a3bc6a63c1e2d0c,
title = "Structural basis of hAT transposon end recognition by hermes, an octameric dna transposase from musca domestica",
abstract = "Hermes is a member of the hAT transposon superfamily that has active representatives, including McClintock's archetypal Ac mobile genetic element, in many eukaryotic species. The crystal structure of the Hermes transposase-DNA complex reveals that Hermes forms an octameric ring organized as a tetramer of dimers. Although isolated dimers are active in vitro for all the chemical steps of transposition, only octamers are active in vivo. The octamer can provide not only multiple specific DNA-binding domains to recognize repeated subterminal sequences within the transposon ends, which are important for activity, but also multiple nonspecific DNA binding surfaces for target capture. The unusual assembly explains the basis of bipartite DNA recognition at hAT transposon ends, provides a rationale for transposon end asymmetry, and suggests how the avidity provided by multiple sites of interaction could allow a transposase to locate its transposon ends amidst a sea of chromosomal DNA.",
author = "Hickman, {Alison B.} and Ewis, {Hosam E.} and Xianghong Li and Knapp, {Joshua A.} and Thomas Laver and Doss, {Anna Louise} and G{\"o}khan Tolun and Steven, {Alasdair C.} and Alexander Grishaev and Ad Bax and Atkinson, {Peter W.} and Craig, {Nancy L.} and Fred Dyda",
note = "Funding Information: This work was partially funded by the NIH Intramural Programs of the National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) (F.D. and A.B.) and the National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS) (A.C.S.). P.W.A. and N.L.C. were funded by Public Health Service (PHS) award A1045741 . Crystallographic data were collected at the SER-CAT 22-ID beamline at the Advanced Photon Source, Argonne National Laboratory (ANL). For the SAXS experiments, we gratefully acknowledge use of the shared scattering beamline 12-IDC resource allocated under the PUP-77 agreement between the National Cancer Institute and ANL and thank Soenke Seifert (ANL) for his support. Use of the Advanced Photon Source was supported by the US Department of Energy, Basic Energy Sciences, Office of Science , under contract W-31-109-Eng-38 . We thank Sriram Subramaniam and members of his laboratory for their preliminary EM efforts. We also thank Nadine Samara for critical reading of the manuscript, Primrose Musingarimi for help with Sf9-expressed Hermes, Robert Hice for assistance with EMSA and insect cell culture experiments, and Susan Chacko of the Helix systems group, CIT, NIH for advice and help with fuzznuc. ",
year = "2014",
month = jul,
day = "17",
doi = "10.1016/j.cell.2014.05.037",
language = "English (US)",
volume = "158",
pages = "353--367",
journal = "Cell",
issn = "0092-8674",
publisher = "Elsevier B.V.",
number = "2",
}