Standardized method for the study of antibody neutralization of HCV pseudoparticles (HCVpp)

Justin R. Bailey, Richard A. Urbanowicz, Jonathan K. Ball, Mansun Law, Steven K.H. Foung

Research output: Chapter in Book/Report/Conference proceedingChapter

7 Scopus citations


Hepatitis C virus (HCV) pseudoparticles (HCVpp) are generated by cotransfection of HCV envelope (E1 and E2) genes along with a retroviral packaging/reporter construct into HEK293T cells. Enveloped particles bearing HCV E1E2 proteins on their surface are released through a retroviral budding process into the supernatant. Viral E1E2 glycoproteins facilitate a single round of receptor-mediated entry of HCVpp into hepatoma cells, which can be quantified by reporter gene expression. These HCVpp have been employed to study mechanisms of HCV entry into hepatoma cells, as well as HCV neutralization by immune sera or HCV-specific monoclonal antibodies.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages10
StatePublished - 2019

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Flaviviridae
  • HCV pseudoparticles
  • Hepatitis C virus
  • Neutralizing antibodies
  • Neutralizing breadth

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


Dive into the research topics of 'Standardized method for the study of antibody neutralization of HCV pseudoparticles (HCVpp)'. Together they form a unique fingerprint.

Cite this