TY - JOUR
T1 - Stable integrative transformation of Trypanosoma brucei that occurs exclusively by homologous recombination
AU - Eid, Josiane
AU - Sollner-Webb, Barbara
PY - 1991
Y1 - 1991
N2 - A calmodulin-neomycin-resistance fusion gene was introduced into Trypanosoma brucei by electroporation, and stably transformed cell lines were obtained. In all of the transformants, the fusion gene had integrated into the host genome at the cognate locus, evidently by homologous recombination within flanking calmodulin DNA. This unusual observation distinguishes trypanosomes as the only eukaryote other than yeast known to undergo gene targeting in essentially 100% of the stable transformants. It should now be possible to systematically manipulate the trypanosome genome, directing predetermined mutations to virtually any chromosomal locus.
AB - A calmodulin-neomycin-resistance fusion gene was introduced into Trypanosoma brucei by electroporation, and stably transformed cell lines were obtained. In all of the transformants, the fusion gene had integrated into the host genome at the cognate locus, evidently by homologous recombination within flanking calmodulin DNA. This unusual observation distinguishes trypanosomes as the only eukaryote other than yeast known to undergo gene targeting in essentially 100% of the stable transformants. It should now be possible to systematically manipulate the trypanosome genome, directing predetermined mutations to virtually any chromosomal locus.
KW - Calmodulin gene
KW - Gene targeting
KW - Homologous integration
KW - Neomvein-resistance gene
KW - Transfection
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U2 - 10.1073/pnas.88.6.2118
DO - 10.1073/pnas.88.6.2118
M3 - Article
C2 - 2006150
AN - SCOPUS:0026060929
SN - 0027-8424
VL - 88
SP - 2118
EP - 2121
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 6
ER -