Splenic imaging with 99Tc(m) labeled erythrocytes: A comparative study of cell damaging methods

R. G. Hamilton; P. O. Alderson; J. F. Harwig; B. A. Siegel

Splenic imaging with ⁹⁹Tc(m) labeled erythrocytes: A comparative study of cell damaging methods

R. G. Hamilton, P. O. Alderson, J. F. Harwig, B. A. Siegel

Research output: Contribution to journal › Article › peer-review

Abstract

Several methods of damaging red blood cells (RBCs) for splenic imaging were compared to determine the optimum approach. The RBCs from donor animals were labeled with (99m)TcO₄^- and damaged by heat, excess acid citrate dextrose (ACD), excess Sn(II) ion, or the sulfhydryl inhibitors N ethylmaleimide (NEM) or p hydroxymercuribenzoate (PMB). The organ distributions of undamaged and damaged RBCs were determined in rats, and splenic imaging studies were performed in rabbits. Splenic deposition and spleen to liver ratios with heat or sulfhydryl damaged (99m)Tc RBCs were significantly greater (p <0.001) than the values obtained using ACD or Sn(II) ion. Heat damaging produces good splenic localization of (99m)Tc RBCs but requires rigidly controlled incubation conditions. NEM damaging provides an excellent and predictable alternative approach.

Original language	English (US)
Pages (from-to)	1038-1043
Number of pages	6
Journal	Journal of Nuclear Medicine
Volume	17
Issue number	12
State	Published - Jan 1 1976
Externally published	Yes

ASJC Scopus subject areas

Radiology Nuclear Medicine and imaging

Cite this

@article{3b327f09aff7472fa9c59138ac464ef5,

title = "Splenic imaging with 99Tc(m) labeled erythrocytes: A comparative study of cell damaging methods",

abstract = "Several methods of damaging red blood cells (RBCs) for splenic imaging were compared to determine the optimum approach. The RBCs from donor animals were labeled with (99m)TcO4- and damaged by heat, excess acid citrate dextrose (ACD), excess Sn(II) ion, or the sulfhydryl inhibitors N ethylmaleimide (NEM) or p hydroxymercuribenzoate (PMB). The organ distributions of undamaged and damaged RBCs were determined in rats, and splenic imaging studies were performed in rabbits. Splenic deposition and spleen to liver ratios with heat or sulfhydryl damaged (99m)Tc RBCs were significantly greater (p <0.001) than the values obtained using ACD or Sn(II) ion. Heat damaging produces good splenic localization of (99m)Tc RBCs but requires rigidly controlled incubation conditions. NEM damaging provides an excellent and predictable alternative approach.",

author = "Hamilton, {R. G.} and Alderson, {P. O.} and Harwig, {J. F.} and Siegel, {B. A.}",

year = "1976",

month = jan,

day = "1",

language = "English (US)",

volume = "17",

pages = "1038--1043",

journal = "Journal of Nuclear Medicine",

issn = "0161-5505",

publisher = "Society of Nuclear Medicine Inc.",

number = "12",

}

TY - JOUR

T1 - Splenic imaging with 99Tc(m) labeled erythrocytes

T2 - A comparative study of cell damaging methods

AU - Hamilton, R. G.

AU - Alderson, P. O.

AU - Harwig, J. F.

AU - Siegel, B. A.

PY - 1976/1/1

Y1 - 1976/1/1

N2 - Several methods of damaging red blood cells (RBCs) for splenic imaging were compared to determine the optimum approach. The RBCs from donor animals were labeled with (99m)TcO4- and damaged by heat, excess acid citrate dextrose (ACD), excess Sn(II) ion, or the sulfhydryl inhibitors N ethylmaleimide (NEM) or p hydroxymercuribenzoate (PMB). The organ distributions of undamaged and damaged RBCs were determined in rats, and splenic imaging studies were performed in rabbits. Splenic deposition and spleen to liver ratios with heat or sulfhydryl damaged (99m)Tc RBCs were significantly greater (p <0.001) than the values obtained using ACD or Sn(II) ion. Heat damaging produces good splenic localization of (99m)Tc RBCs but requires rigidly controlled incubation conditions. NEM damaging provides an excellent and predictable alternative approach.

AB - Several methods of damaging red blood cells (RBCs) for splenic imaging were compared to determine the optimum approach. The RBCs from donor animals were labeled with (99m)TcO4- and damaged by heat, excess acid citrate dextrose (ACD), excess Sn(II) ion, or the sulfhydryl inhibitors N ethylmaleimide (NEM) or p hydroxymercuribenzoate (PMB). The organ distributions of undamaged and damaged RBCs were determined in rats, and splenic imaging studies were performed in rabbits. Splenic deposition and spleen to liver ratios with heat or sulfhydryl damaged (99m)Tc RBCs were significantly greater (p <0.001) than the values obtained using ACD or Sn(II) ion. Heat damaging produces good splenic localization of (99m)Tc RBCs but requires rigidly controlled incubation conditions. NEM damaging provides an excellent and predictable alternative approach.

UR - http://www.scopus.com/inward/record.url?scp=0017101936&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017101936&partnerID=8YFLogxK

M3 - Article

C2 - 993833

AN - SCOPUS:0017101936

SN - 0161-5505

VL - 17

SP - 1038

EP - 1043

JO - Journal of Nuclear Medicine

JF - Journal of Nuclear Medicine

IS - 12

ER -

Splenic imaging with ⁹⁹Tc(m) labeled erythrocytes: A comparative study of cell damaging methods

Abstract

ASJC Scopus subject areas

Other files and links

Fingerprint

Cite this